2A (NC_013114) Virus Tagged ORF Clone

CAT#: VC100708

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 98], codon optimized for human cell expression, YP_003104779

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100708 represents NCBI reference of YP_003104779 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCATTTGGTCAGCAGTCAGGCGCTGTTTACGTGGGGAATTACCGCGTGGTTAACCGGCATCTGG
  CCACGCATAACGATTGGCAGAACTGCGTCTGGGAGGATTATAACAGAGACCTCCTTGTTTCCACAACTAC
  AGCCCACGGGTGTGACACCATCGCTAGATGCCAGTGCTCTACCGGCGTTTACTTCTGTGCCAGCAGAAAT
  AAGCATTATCCAGTTAGTTTCGAAGGCCCTGGTTTGGTTGAAGTGCAGGAGAGTGAGTACTACCCTAAAC
  GATATCAGTCTCATGTCCTGTTGGCAGCTGGATTTTCCGTGCCAGGGGACTGTGGTGGCATTCTCCGATG
  TGAGCATGGAGTTATCGGGCTTGTTACAATGGGCGGAGAAGGAGTAGTAGGGTTCGCTGATGTCCGGGAC
  CTCCTTTGGCTGGAGGACGATGCAATGGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100708 representing YP_003104779
  Red=Cloning sites Green=Tags
  
  MGAFGQQSGAVYVGNYRVVNRHLATHNDWQNCVWEDYNRDLLVSTTTAHGCDTIARCQCSTGVYFCASRN
  KHYPVSFEGPGLVEVQESEYYPKRYQSHVLLAAGFSVPGDCGGILRCEHGVIGLVTMGGEGVVGFADVRD
  LLWLEDDAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 453 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104779
• RefSeq ORF: 453
• MW: 16.6 kDa