2C (NC_013114) Virus Tagged ORF Clone

CAT#: VC100710

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 98], codon optimized for human cell expression, YP_003104781

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100710 represents NCBI reference of YP_003104781 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACGGCTGGCTGAAGAAATTTACAGAGATGACGAATGCTTGTAAAGGAATGGAATGGATCGCAA
  TCAAAATCCAAAAGTTTATCGAATGGCTGAAAGTCAAAATATTGCCCGAAGTTAAGGAAAAGCACGATTT
  TCTGAACCGCCTTAAACAGTTGCCCCTGCTGGAATCTCAAATCGCCACCATCGAGCAGTCAGCTCCAAGC
  CAGAGCGATCAGGAGCAGTTGTTCTCAAACGTTCAGTACTTTGCTCATTATTGTCGCAAGTACGCTCCCC
  TGTACGCCGCTGAGGCCAAACGGGTGTTCAGTCTGGAGAAGAAGATGAGCAACTATATCCAGTTTAAGTC
  CAAGTGTCGCATCGAACCGGTCTGCCTCCTCCTCCACGGCTCACCGGGGGCTGGGAAATCCGTGGCTACA
  AATCTCATTGGGAGATCCCTCGCAGAGAAACTGAACTCATCTGTGTACTCCCTGCCTCCAGACCCGGATC
  ATTTCGACGGATACAAGCAGCAGGCGGTGGTCATCATGGATGATCTCTGCCAGAATCCAGATGGCAAGGA
  TGTGTCTTTGTTCTGTCAAATGGTAAGCTCTGTGGATTTCGTGCCCCCGATGGCAGCCCTGGAGGAAAAG
  GGGATCCTTTTTACAAGCCCGTTTGTCCTTGCATCCACCAACGCCGGCTCTATCAATGCCCCCACTGTCT
  CCGATTCCCGAGCCCTGGCTCGCAGATTTCATTTCGACATGAACATAGAAGTGATCAGTATGTACAGTCA
  GAATGGCAAAATTAACATGCCGATGTCAGTTGAAACGTGCGACGATGAGTGCTGTCCCGTTAATTTCAAG
  AAGTGTTGCCCTCTGGTTTGCGGCAAAGCAATCCAATTCATCGACAGGCGCACACAGATGAGGTATTCTT
  TGGACATGTTGGTGACAGAGATGTTTCGCGAGTACAATCACAGACACAGTGTTGGGGCTACCTTGGAGGC
  TCTGTTCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100710 representing YP_003104781
  Red=Cloning sites Green=Tags
  
  MSNGWLKKFTEMTNACKGMEWIAIKIQKFIEWLKVKILPEVKEKHDFLNRLKQLPLLESQIATIEQSAPS
  QSDQEQLFSNVQYFAHYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVAT
  NLIGRSLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMAALEEK
  GILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPMSVETCDDECCPVNFK
  KCCPLVCGKAIQFIDRRTQMRYSLDMLVTEMFREYNHRHSVGATLEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013114
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013114.1, YP_003104781
• RefSeq ORF: 990
• MW: 37.4 kDa