U14 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101554

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL35 [Human herpesvirus 7], codon optimized for human cell expression, YP_073755

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101554 represents NCBI reference of YP_073755 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGCAGAAGGGTTTCAGTATTCCATTTTTTGTGACCGATGAAAACTGTAATTTTGTGCCCGAGA
  TTCTCCCCAGGATCCACACAAAGTTTCTTAAGGATGTTCTTATCGCTGATTCTTACAACAGCGTCAGCTG
  GGCTAACTCATTCATCCCTATGCCCATTCAGACCCTGGAACAAATCATGGTACTCATAACTAAGTTTAAA
  TTCTCCCGAAGCCGCGATTTCTTGTTCCCAGTTATCCGGTTGGCTGTGCATATCAACCGGTTTCACACAG
  GGAAAAAGCAACTGAAAACAATGATCGAGATTATGAAGAGCCTTTTTAACACAGAGGAAGCAATGAGACG
  CTTCGACGAGGCGCTGATGATACTGTTCTCTAATGAGCAAACCAACACGTACATGACCAACATCGCGCTG
  TCTATGCATGAGAATGGACTGCCTGATTCAAAATTTATGAATGCACTGAAAATGATTTACCGCGCAGGTA
  ATTCATTCGACAACCAGCCTGACAACGATATCGAATCTTACAACGAGAAACTCAAGATCTATAATTATCT
  TATTAAGATACCTAAATATACACTCAAAGCCGGGGTGGATCTCTATAACGAGAACATCAAAGATCTTTCA
  ATCGGTATCCAGCGCCAGCCGACGCTGCTTTTTACTTCACGGAACGATTTTTCACTTAAAGCTATCTATA
  ACGACGTGCTCTTCCTGGTCAGTGCTTGGAACATGATCATAAACTACAAAAAGGAGCAACGACGGCTCTT
  TTCTTGGATTACATTCGAAATCAACTCTCTGATGGAGAACGTCGTCCTGGCCGCCTTTCAGCTGCCAGAT
  TTGAAAGAAATGACGTTGGATCTGTCAGCTTTGATCGCCAACATGAACCTTTTGAAACCCAATGACGACT
  ATTCCCCGCACTTCAAATTGATCATCAACAAGTTCTTTGAGATTGGAATCTTTGTTACAAAATCCTATAT
  TTGTATCCTGCCCAGCTTTGTTAAGTCACAACTGATCTCCTTCGAAAACGTCCTGTCATCCAACAGACAC
  GCCGAAGATGTGACGTTTATCCTGACTAGTTCCAAAGAGTCCGACGACGAATATGACGAAGATAAACCAC
  CCAGGCAGGTTGACCCTGACAGGGTCGATAATATTCTCATGGAAAGCGATTTCTTTAACGTGAAGCCCGA
  GAATGCCTTTTCTGAAATAGCACTGATGCCCATCTCCCATGACAAGATCATCGATGTGAACAACAGTAAT
  ATTCAGGTATTGGAAACCGAACTGGCGCACACCAACCTTTTCGTGTACTCCGCCATTGCCCAGAAGTACG
  ATCTGCCCTTGAAGGAGTACGTTGAGCGATTGAATGTATATAATCCAGATCTGTCATCCGGAAACTCCAC
  CCCTGCGAGAAATTCCAACAGCATTAGGACCACGCCGGTGCTTAATATCTCCAGACCCGGCTCAACAACC
  CCTAGTGGCAATTCCGCCAGATATGGCAATAATACTCCTCGGAGTATCACGCCTGTGCTCGAGATTAGTC
  GCTCACGGAGCGCTACTCCATCTGGGAACTCCGAAATATACGAGAATCGGACAAGCCCTACGTTCCGGGT
  GTCTCGCAGCGCCACCCCAATAGAAAGGAGCAGCAGGTCAGCAAGCATAATAAGCGGCGAGTCAGTGCCC
  GGGTTCTTCAACGATCAAGAACGGCTGTCCACAAATTCCCCTATTAGTATTAACGGAAACACCCCGAGAC
  AACAGTCCCATGGGGACAACGAAATTCAGACCATTGATTCCACCGACGAAGACAGCATGAATGCTCCACA
  ATCTCCTCAGTCCATCTACAGTATCTCCTCATACGTCTCCACAGACGATCAACTCCTCCACTCCCCTACT
  AATAGTCCATTTAATCTGTTCGATTCCGTGTCCGAGATGCAAGAAGACACCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101554 representing YP_073755
  Red=Cloning sites Green=Tags
  
  MEQQKGFSIPFFVTDENCNFVPEILPRIHTKFLKDVLIADSYNSVSWANSFIPMPIQTLEQIMVLITKFK
  FSRSRDFLFPVIRLAVHINRFHTGKKQLKTMIEIMKSLFNTEEAMRRFDEALMILFSNEQTNTYMTNIAL
  SMHENGLPDSKFMNALKMIYRAGNSFDNQPDNDIESYNEKLKIYNYLIKIPKYTLKAGVDLYNENIKDLS
  IGIQRQPTLLFTSRNDFSLKAIYNDVLFLVSAWNMIINYKKEQRRLFSWITFEINSLMENVVLAAFQLPD
  LKEMTLDLSALIANMNLLKPNDDYSPHFKLIINKFFEIGIFVTKSYICILPSFVKSQLISFENVLSSNRH
  AEDVTFILTSSKESDDEYDEDKPPRQVDPDRVDNILMESDFFNVKPENAFSEIALMPISHDKIIDVNNSN
  IQVLETELAHTNLFVYSAIAQKYDLPLKEYVERLNVYNPDLSSGNSTPARNSNSIRTTPVLNISRPGSTT
  PSGNSARYGNNTPRSITPVLEISRSRSATPSGNSEIYENRTSPTFRVSRSATPIERSSRSASIISGESVP
  GFFNDQERLSTNSPISINGNTPRQQSHGDNEIQTIDSTDEDSMNAPQSPQSIYSISSYVSTDDQLLHSPT
  NSPFNLFDSVSEMQEDTE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073755
• RefSeq ORF: 1944
• Locus ID: 3289473
• MW: 73.9 kDa