U70 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101610

Myc-DDK-tagged ORF clone of viral ORF for deoxyribonuclease [Human herpesvirus 7], codon optimized for human cell expression, YP_073810

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U70
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101610 represents NCBI reference of YP_073810 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATTGACTACGCCCAGATCTCCTGCAACCTGGCCTCAATCATCGAAGAGGACAGTGTGTTCTTGT
  TTCTGATCGATAAATTGAACAACCTGGATATCTCAAGAAGGAAAATCTCCTTTAACTTTATCAGACTGTG
  CTACACATACTATATCTTGATTAAGTTCAACAGCAGGTTTAAGGATACTTTTCTCGCCAGGTCATTTATT
  GATTATATGCACCAAAATATTAGCGATTTTATTGACGAGAACGTTGAGCTGAGCGACCTCTATTCCAATA
  TCTACGTACGACTTCAGGATGCAAGTCCTAAGGTTGTGAAAAACCTGTTCAAGATTCTGGAGCGGGAAAC
  AAGGGGGCAGAGCACCAACCCATTGTGGCACGCCATGCGGAAGAACTGTATAACAGCGACGAAGATTTAT
  GACATTTACATCTCTAAAAGCTTTTCCGGCATACAGGAACACTCCTATCTGGGGGACGCAGTGCTCTATG
  GAATTAAGCATGAAAGGATTATCGAGCATCTCCTGAAGACTTTTTTCGTGAAGAAGCCGTGGATCAGCAA
  GACACTTGGACTTTTGCTCGACCCCTCCTCCGGCGTGTTCGGTGCTTCCATCGACTCCTACTATGGCATT
  TCTTTCAATGACAATAACCTGATTGAGGTGGGTGACAAGGTGGTGATCTTTGAACTGAAATTCCGCTATA
  AGTATCTTCGGGAGAAAAATGACCTCTTTGTCTCAGAGTTGCTTCAGAACCCTAGCGAAATTGCACTCGC
  TAAGTTTATCCTCTCTCATCCGATTCCAGCCATCGAGTACCGGGAGAACGGTAAGATGCCCAGTGCCCGA
  GAGTATCTGATAACAAACAATCCCCTCTATGACAGCGGCAAGAAACGCAGGGCATGTCTTACCCCTAAAA
  ACCTCACATTCGATATCACCAGGTTGATCCCAATGAACGAAAAAAATGTCTCTACTGCCATCATCTTCGA
  CGTGGTCAAGGACTGTATTCTGAACACCCTGGTGGCCTATCAGAAGGCCATCTTCACAATTGACGCATTC
  ATCAACCCTAGACACAGGTATTACTTCCAATCCATCCTGCAACAATATGTGATGACGCAGTTTTACATTC
  AGGATCATGATAACCCTGAGAATATTGAAAAGGAAAACCTCCCCTCCGTGTACATCGTTAGTGCCATATT
  TCGCAAACGGGAGGATGATGAAAAGAACTGCAGGCTGCTGATCGAAGATACGGAGTACCTCGAGGAGGAG
  ATCCCACTGATCCTGCTTATAACTCCCATCACCATCGATGCAGAATTCACAAGCCGCGTCATCAAGGATA
  TCTGCTGTATCTGGGAGAATAAGATAGCTCAACAAACAAATTTGAAGATCTGGGCTCAATCAGCTGTCCG
  CCAGTACATGGCCGCGAGTAGCGCGCGACCGAAGACCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101610 representing YP_073810
  Red=Cloning sites Green=Tags
  
  MAIDYAQISCNLASIIEEDSVFLFLIDKLNNLDISRRKISFNFIRLCYTYYILIKFNSRFKDTFLARSFI
  DYMHQNISDFIDENVELSDLYSNIYVRLQDASPKVVKNLFKILERETRGQSTNPLWHAMRKNCITATKIY
  DIYISKSFSGIQEHSYLGDAVLYGIKHERIIEHLLKTFFVKKPWISKTLGLLLDPSSGVFGASIDSYYGI
  SFNDNNLIEVGDKVVIFELKFRYKYLREKNDLFVSELLQNPSEIALAKFILSHPIPAIEYRENGKMPSAR
  EYLITNNPLYDSGKKRRACLTPKNLTFDITRLIPMNEKNVSTAIIFDVVKDCILNTLVAYQKAIFTIDAF
  INPRHRYYFQSILQQYVMTQFYIQDHDNPENIEKENLPSVYIVSAIFRKREDDEKNCRLLIEDTEYLEEE
  IPLILLITPITIDAEFTSRVIKDICCIWENKIAQQTNLKIWAQSAVRQYMAASSARPKTP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073810
• RefSeq ORF: 1440
• Locus ID: 3289528
• MW: 56.0 kDa