U84 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101621

Myc-DDK-tagged ORF clone of viral ORF for protein UL117 [Human herpesvirus 7], codon optimized for human cell expression, YP_073821

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101621 represents NCBI reference of YP_073821 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGCAAGAAACGAAAAACGGTATGTAAAGCTAAGGGCAAACTTACGAGCAAGTCCTGTATGTGTA
  CTAAGGCTAACGCTCGCTACACCTGCAACTGTTTCAGCAAGACCCTGCCATTTAACGAAAAGGCAATAAA
  GTGCACCATCCCTGAAAAGATCAACTCCGAGATAAATATCTCTAAATCTGAAATGCTCCTGAAGGCGGAC
  AAATATACCAATGCCATATACAAGCATTCTAAACTGAGGAAAATTTTTAAGGCTGTGAAAGAGAATCTGT
  CAACCACTAATTTCGCAACAAAGAGCAAAGGAATAGTGCATTCAAACTTTAACGATTCAAAGCCAATCCA
  CTCACCTGAGATTAAGAATTATACAGATTTTAATTGTGATTTCATGCATCCATGCGGTCTCTGTAGTCCA
  AAAAATTTTCTGTCCGAGGTGTACAAACTTCTTCGCTTCTATAACTCTACAGTGGTTCAGATCCAGGTGT
  ATAGCCGGAACGGGCTTCTCGTGAATAGTCTGCACGAGAAGTTGATGGAAATCGCCAACCTGATCGTTAA
  TAAAGGTGAACAGACCGTGTCAGAGATCTTTTTCCACGAACAGAAGACCGCCATCGCCATTAATCGGTTC
  CTCCGCGGGACTGTCCCCCATACAGAGTTGACCCGAAAAGAGGCAATGATGCCGGTGAACGTTAATAGCA
  AGCATCGGAAAAACTCCGGCGTGTACTCACTCTACATTAACGCTCCGACCGAGAAAGACTTGTTCTGCGC
  TGCTCATTTGTGTGTTCAGTTCTCTATACGCTATCCTGCAGACATCCTTTTGAACGTATCCAAATTGACA
  CAGCCTCTTCTGCTGAAGGAACACCTTATTATCTACAATACCTTTAACCGCTACATCTATTGGGACGATT
  ATGGGAAGCTCCTGAAGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101621 representing YP_073821
  Red=Cloning sites Green=Tags
  
  MRGKKRKTVCKAKGKLTSKSCMCTKANARYTCNCFSKTLPFNEKAIKCTIPEKINSEINISKSEMLLKAD
  KYTNAIYKHSKLRKIFKAVKENLSTTNFATKSKGIVHSNFNDSKPIHSPEIKNYTDFNCDFMHPCGLCSP
  KNFLSEVYKLLRFYNSTVVQIQVYSRNGLLVNSLHEKLMEIANLIVNKGEQTVSEIFFHEQKTAIAINRF
  LRGTVPHTELTRKEAMMPVNVNSKHRKNSGVYSLYINAPTEKDLFCAAHLCVQFSIRYPADILLNVSKLT
  QPLLLKEHLIIYNTFNRYIYWDDYGKLLKT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073821
• RefSeq ORF: 930
• Locus ID: 3289539
• MW: 35.7 kDa