U47 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101586

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein O [Human herpesvirus 7], codon optimized for human cell expression, YP_073787

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101586 represents NCBI reference of YP_073787 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACAAGATGTACTCCATGCTCGTGTTCACTCTTATATCATTCTTGTTTTACTCCTACCTGATTA
  TCTGGACCCCCGTGCTTTCAGTATGCTCCGAGAAGATCTCTTTTGTGAATTTGACTAATTTTAGTATGTG
  GCCCAAGTTTGCAAAGTTTCACTACGAGAGTTTTGAGAAAACATACATCCAATCCTGCGATATCCCGATC
  TCAAAAAACTGCTTTAAGCAGATCCTTTTTTGGTCCTTTCGGTTGAGTCAAAAGAAAAACATTTGCCTCT
  CTAAGATTAATATGCTGTATCTGGATAACTTTCCTCGCTGGACACTGCACTTCGAGTTCCCCACCAAGAC
  AAATCGGAAAAGACACGTGTACCTCGACAACATTTCCTTTCTGTACCTGGTGTTCGCCACTCTCGCGTTC
  AAGACAATGGACGATTCATGTGTTAACAAAATCCCCTTTGAGGTCCTGGCTTCTCACTTGTTTAAGATCA
  ACTTCTCACAGAACAAAATCGAGACAATCTTCCAGGACCTCCAGAACCACACTGAAGCACAGCTGTTTTC
  CAGGGAAAATTCCGAGCAGCTGTTTAGCTTTACAAATTTCATTTACTATTTTGTTTACAATAGGACAGAC
  TGCAAAAATTCTATTAGTAAGTTTTTTTACAACAGCGTTAATACAAGAAACGTGTCTACACCATTTGGCG
  TCACTAATTTCTCTCTGGTCAGAGGTATCATGTCACCCATTCAGAGCTTCAAGGGTAATCTTATGTTTCT
  GGAGAACAAGACAAAGGTCACAAAGCCGACCGCCATTCCTAACGCCGTGACTAGCGAGCCAACAAAGTTT
  TTCCCTAGTACACGGGGCACCAGCTCAATGCAAGCAAGCCAGCAGACATCTAGTTTCCCTACAACTTTCT
  TTACAACCAATCACTCAAATACATCCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101586 representing YP_073787
  Red=Cloning sites Green=Tags
  
  MKNKMYSMLVFTLISFLFYSYLIIWTPVLSVCSEKISFVNLTNFSMWPKFAKFHYESFEKTYIQSCDIPI
  SKNCFKQILFWSFRLSQKKNICLSKINMLYLDNFPRWTLHFEFPTKTNRKRHVYLDNISFLYLVFATLAF
  KTMDDSCVNKIPFEVLASHLFKINFSQNKIETIFQDLQNHTEAQLFSRENSEQLFSFTNFIYYFVYNRTD
  CKNSISKFFYNSVNTRNVSTPFGVTNFSLVRGIMSPIQSFKGNLMFLENKTKVTKPTAIPNAVTSEPTKF
  FPSTRGTSSMQASQQTSSFPTTFFTTNHSNTST
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073787
• RefSeq ORF: 939
• Locus ID: 3289505
• MW: 36.7 kDa