U82 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101620

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein L [Human herpesvirus 7], codon optimized for human cell expression, YP_073820

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101620 represents NCBI reference of YP_073820 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTAATATCTTCTTCATATTCTTGATTAGCATACTCAATCAGATTTACGCTCTCTTTAACAACT
  CATACTACTCAAATCTCGAACAGGAGTGCATCAAGAATATTCTCAACTGTACCCAATCCAAGACCCTTAG
  TCTGCTGGAGCCCATCGATCAGGCTCCCATCCCAAATTCAGATATCATATCTAGGCTGTTGTATCACACT
  CCCTACATATCCAGAAGGGATCAGGTATTGATAGATGAGGACTTTTTGGAGACATTTTATCTGCTCTACA
  ACAATCCCAATCAACTGCATACACTGCTCAGCCTTATTAAAGACAGCGAATCTGGCCATAATTGGCTCGG
  CTTTCTCAACAACTTTGAGCGATGCCTTTCAGACAATACCCTTCTGACCTGTCGCGACAATGTCTGCAAA
  TCCTACAGCTATGAGAAGCTGAAATTCACTGGAAATATCTTCGTGGAAAACATCATAGGCTTTGAATTCA
  ACATTCCCTCCAACATGATTAATTTTAACATGTCCATCCTGATCTATTTGGAGAACGAGGAGACAAGGAC
  GCAGAGGATAGTACGAATCGACCACCATGGCATTAATGTTTTTGACGCCCTGCTGAATTGTCTCCGGTAC
  TTCTCTAGGTACTATAACTTTTCCTTTCCGCTCATTCAGGAGATGGAGAAATACAATGAAGTCCTTCCTT
  TTAGGTCCGAATTCTCTAACCTGCTGATTCGCACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101620 representing YP_073820
  Red=Cloning sites Green=Tags
  
  MKTNIFFIFLISILNQIYALFNNSYYSNLEQECIKNILNCTQSKTLSLLEPIDQAPIPNSDIISRLLYHT
  PYISRRDQVLIDEDFLETFYLLYNNPNQLHTLLSLIKDSESGHNWLGFLNNFERCLSDNTLLTCRDNVCK
  SYSYEKLKFTGNIFVENIIGFEFNIPSNMINFNMSILIYLENEETRTQRIVRIDHHGINVFDALLNCLRY
  FSRYYNFSFPLIQEMEKYNEVLPFRSEFSNLLIRTY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 738 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073820
• RefSeq ORF: 738
• Locus ID: 3289538
• MW: 29.2 kDa