U27 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101566

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase processivity subunit [Human herpesvirus 7], codon optimized for human cell expression, YP_073767

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101566 represents NCBI reference of YP_073767 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGATCTAACCGGGAACACCACTCACATAGGGATCACAGAGAACATCGCGACTCCAAAGAACCTC
  CAACCATGGCCTTCCATATGAAAACGTGGAAGACGATCAACAAACCTCTCAGGGCCTTCGCGAAGCTTCT
  GAGAGAGAACACAACCGTTACCTTTACACCCCAACCAGCAATTATAATTCAGAGCGCCAAGAACCATCTG
  GTTCTGAAACTCATTATTCACGCAGAGTGTCTTTACATAACCGATACTGACCATTTTTCTACTAAGACAA
  TAAATAACTTTGTTCCTTTGTTCGATTCCTTCATGGGAATAATCAGCAATCCCGATGTGACTAAACTGTA
  TATTCAGCACGACTCTGATCTGTATACTCGATTTCTGGTCACTGCTTCCGACATTTGTGCGCAGGCGAGT
  ATCCCCTGTGTAAACGGCCAGGAAATAGTGAGAGAGAGTGGGAAAAGCGCACTGAGGATCGATCTCGACC
  ACTCCACCGTTACGGAGATCTTGAAATGGCTGGCCCCCGTAACCAAGAACAAGCGATCCAATAAGAACGA
  GATGACACTCGCTCAGATAGTTGTGCAGGTCAACCCGCCTTCTATCAAATTCCTGACAGATCTTAATGAG
  ATTGAATTCGCTCATTCTGGGAAGGTGGTATTCCATGACGCAAAATGTATGCGCCTGGTTCTGTCATCCA
  AGAACCTGCAGCAGGCCTTCAGCACGTGCGCGGTGCTCAAGTCCAGTTGTTCCCTGCGGGCAATTGCCGG
  GAAAGAATACAAATTGTTTCTGATAGCCAAGAATGTTTTCCTCACAGTGGAGGCATATCTGTCCCAGGAA
  CAGGTCAAGGACGATCCGAAGTTTGAGAGACAGGCCAAGACCGAAGAGAAGGGGGAAAAAAACCATAAGG
  TTGAGGAAGGTAATAATTTCTTTTGTAAGCAGGAGACCCAGCACAAGATTACCTCCTACATGGTGCCCAC
  CAAAAATGGAGGAACGGGCACAAACTTTTTTAACGAGAAAGAAGATTCTGAGTCTGACGATAGTGCTCAC
  TTTGACTACACACCCAACTCCAAACGCCAGCGGTGCGGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101566 representing YP_073767
  Red=Cloning sites Green=Tags
  
  MDRSNREHHSHRDHREHRDSKEPPTMAFHMKTWKTINKPLRAFAKLLRENTTVTFTPQPAIIIQSAKNHL
  VLKLIIHAECLYITDTDHFSTKTINNFVPLFDSFMGIISNPDVTKLYIQHDSDLYTRFLVTASDICAQAS
  IPCVNGQEIVRESGKSALRIDLDHSTVTEILKWLAPVTKNKRSNKNEMTLAQIVVQVNPPSIKFLTDLNE
  IEFAHSGKVVFHDAKCMRLVLSSKNLQQAFSTCAVLKSSCSLRAIAGKEYKLFLIAKNVFLTVEAYLSQE
  QVKDDPKFERQAKTEEKGEKNHKVEEGNNFFCKQETQHKITSYMVPTKNGGTGTNFFNEKEDSESDDSAH
  FDYTPNSKRQRCGM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073767
• RefSeq ORF: 1092
• Locus ID: 3289485
• MW: 41.6 kDa