U37 (NC_001716) Virus Tagged ORF Clone

CAT#: VC101576

Myc-DDK-tagged ORF clone of viral ORF for nuclear egress lamina protein [Human herpesvirus 7], codon optimized for human cell expression, YP_073777

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101576 represents NCBI reference of YP_073777 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAATTCAAAGTACCAGGCGCTTGCGGCGCGCATCTTCTTTGTTGAAGAAAAGTAAGCCGTATAATA
  AAGAGAAGACCAATCTGTCACTCTCTCTCTCTCTTAAAGAACTGCACAGTGTGTTTAAGCTCTTCCCTGA
  GTACGAGCTTAAGTTCCTCAATATGATGAAACTTCCAATCACAGGCAAAGAGCCTATCAAGATCCCCTTC
  GACTTGAGTCTGCATCACCAGCACACATGCCTGGATCTCAGCCCCTACGCCAATGAGCAGGTCAGCAAAT
  CCGCATGCGTGAATTGCGGCACCACTAATATCCCTACGGCCTCCGATGCCATGGTGGCCTACATGAACCA
  GATTTCCAACGTTATGCAGAACAGGTTGTATTATTACGGTTTCCAGAAAAAGGTGGAACTGATTCGCATG
  TCAGCCAAGCAGCCCACACTTTTCCAAATTTTTTACATCCTCTCTTCCATTGCCAGCAATTTTCTTCCGA
  TAATGTTCGAGAATAACGAGAAGCTGAACATGTATGTGGTGTTCCAGACAAGGACGCTGCACATCCCGTG
  CGAGTGTATCAATCAGATCATGACGGTGAGCAGCGGATACACAGTTTTGTTGGACATTCTGCACGATTCC
  ATCGTCTTGCACGTACTGTGTAAGACAATCGAAACCTCAAACATTCAAATTGACATCAACGTGTTGCAGA
  GGAAGATTGAAGAGATGGATGTGCCCGACGAGATCGGTGACAAGTTCGAAAAACTGAAGCATATTTTGCC
  ATTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101576 representing YP_073777
  Red=Cloning sites Green=Tags
  
  MIIQSTRRLRRASSLLKKSKPYNKEKTNLSLSLSLKELHSVFKLFPEYELKFLNMMKLPITGKEPIKIPF
  DLSLHHQHTCLDLSPYANEQVSKSACVNCGTTNIPTASDAMVAYMNQISNVMQNRLYYYGFQKKVELIRM
  SAKQPTLFQIFYILSSIASNFLPIMFENNEKLNMYVVFQTRTLHIPCECINQIMTVSSGYTVLLDILHDS
  IVLHVLCKTIETSNIQIDINVLQRKIEEMDVPDEIGDKFEKLKHILPFI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001716
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001716.2, YP_073777
• RefSeq ORF: 777
• Locus ID: 3289495
• MW: 29.9 kDa