E6 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101789

Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084346

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101789 represents NCBI reference of YP_003084346 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCCATTCCCCAATGATCTCAGGTCATACTGTAATTACTTTGGCATATGCCTCTTCGACCTCA
  GACTTCAATGCATCTTCTGTAAGAGCATTCTCGATATCGTGGATCTTGCTAAATTCCATAAGAAAGAGCT
  TAGGCTGGTGTGGAGGTGCAAAGTGGCATACGCATGTTGCAGCAAATGTCTGTACGCTTCAGCACGGTAC
  GAGAACGAGAATCATTTCCAATGCGCAGTGAAAGCTAGCACCCTTCATGACCTGCTGGGGACTCCCCTGC
  ACCAAATTTACATGCGCTGCAACCATTGCCTCTCAGGGCTTGATCTCCAGGAGAAATTTGACCTGGTAGC
  TCGGGATTGCTATGTAATCTTGGTGAGGGGGTACTGGCGCGGGCCGTGTAGAGACTGTATAAATAGAGAG
  TAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101789 representing YP_003084346
  Red=Cloning sites Green=Tags
  
  MENPFPNDLRSYCNYFGICLFDLRLQCIFCKSILDIVDLAKFHKKELRLVWRCKVAYACCSKCLYASARY
  ENENHFQCAVKASTLHDLLGTPLHQIYMRCNHCLSGLDLQEKFDLVARDCYVILVRGYWRGPCRDCINRE
  Y
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084346
• RefSeq ORF: 423
• Locus ID: 8223424
• MW: 16.6 kDa