E2 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101792

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084349

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101792 represents NCBI reference of YP_003084349 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAGAGCGACCTTACAGAGAGACTCGATGCTCTTCAGACTGCTCTTATGAACATTTACGAAGAGG
  CCCCTACCGATCTGCCATCACAAATTAGGCACTTCGACCTCCTGAGGAAGCAGAGCGTCCTGGAATACTA
  CGTCCGGAAGGAAGGCTATACACAACTCGGACTGTATCACATCCCAACGCTCAAGGTTAGTGAGTATCAT
  GCAAAGGAGGCCATTAAGATGGGTATTGTTCTGAGAAGCCTGCAGAAGAGTCCCTATGCAGACGAAGAGT
  GGTCTTTGCAGGATGTTAACGCTGATTTCTTTAATAGTCCACCACGCAATTGTTTTAAAAAAGGAGGTTA
  CGATGTAGAGGTTTGGTTCGATCACAATCCCCTGAACACCTTCCCCTACACTAATTGGACTTGGATTTAC
  TATCAGGATGACGAAGAGAATTGGCATAAGGTGCAGGGCGAAACCGACTATAATGGGCTTTTTTATAGAG
  AGACGGATGGCACCGTTGTGTACTTCCTGCTCTTTGAGTCTGATGCAGCAAGATACGGCACTAAAAATGA
  GTGGACCGTAAATGTTAAAAACGAACAGATTAGTCTCCCTGCCAATTCTAACGGCAGGAGGTCTAGTAGC
  GGTACACCGACACACTCCACGACTAACAGCGTTGCTGAGCCTGGACCTAGCAGGTCCACAGAGGAGGCCG
  ATGGACGACGCACACCTCAAGCGCAGACCCAATCAGTAGGCGCATCCAAGAAGCCTTCATCCGTCGGCCG
  ACGCAGAAGGCGGAAGCAAGGCAAGCAAACTCCTAGCAAACGCCGCCGGACTGGTGGAGGCTCTGGTGAT
  GAAGCAGACTCCGGAGGGATTTCTGCAGAAGAAGTGGGATCTTCACATCGATCCGTGGCAAGATCCGGAC
  TGAGCCGACTGGAAAGACTCCAGAAAGAGGCCCGGGACCCCTACATCATCCTGGTTAGAGGGCCACAGAA
  TACCCTCAAGTGTTGGCGCTATCGCATTCAAACTAAGGCAAATTTTCCATTCCTGTACATTAGCACTGTG
  TGGAAGTGGGTCACTAAGGATGCTGTGGGACACGAGGGTCGCGTACTTATTGCCTTCTTGAGCAAGGAGT
  CCAGAGACCTTTTCGCCAATTCCGTTCACTTTCCCAAGAACACTACCCACAGTTATGGATCTCTCGATGC
  ACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101792 representing YP_003084349
  Red=Cloning sites Green=Tags
  
  MNQSDLTERLDALQTALMNIYEEAPTDLPSQIRHFDLLRKQSVLEYYVRKEGYTQLGLYHIPTLKVSEYH
  AKEAIKMGIVLRSLQKSPYADEEWSLQDVNADFFNSPPRNCFKKGGYDVEVWFDHNPLNTFPYTNWTWIY
  YQDDEENWHKVQGETDYNGLFYRETDGTVVYFLLFESDAARYGTKNEWTVNVKNEQISLPANSNGRRSSS
  GTPTHSTTNSVAEPGPSRSTEEADGRRTPQAQTQSVGASKKPSSVGRRRRRKQGKQTPSKRRRTGGGSGD
  EADSGGISAEEVGSSHRSVARSGLSRLERLQKEARDPYIILVRGPQNTLKCWRYRIQTKANFPFLYISTV
  WKWVTKDAVGHEGRVLIAFLSKESRDLFANSVHFPKNTTHSYGSLDAL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084349
• RefSeq ORF: 1194
• Locus ID: 8223420
• MW: 45.4 kDa