putative E4 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101793

Myc-DDK-tagged ORF clone of viral ORF for putative E4 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084350

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: putative E4 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101793 represents NCBI reference of YP_003084350 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAAATGAACAAATCCCCCTTTCTCCCAACCGCCACAGTGGGGGGATTGCGCTTGGGTCCCCAGC
  TGACCCTTCCGCCGACTCTCAGCCTTAGTAGAGGGCCACCAGACCCGCCCCGCAGACCGACAGACGAGGA
  ACGACACAAGCACCGAAGGCGAGCCCTGGGACTCCCAAAAAACCATTTGCGCAGTGACGACGAAGGAGAC
  GAGAACAAAGAAAACAAGCCTCCTGCTAACGAGGATGAACTCGAGGAAGAAGCTGAGACTAAGCAAACTG
  CTGTGGAATCTCTTCTGCGGAAGCTTGAGGTGGCTATAGATCAGTTGCATGATCAAGTGTACAGAGACTT
  GAACGACTTTAAGAGAAGGCTGGGCATAAGAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101793 representing YP_003084350
  Red=Cloning sites Green=Tags
  
  MLKMNKSPFLPTATVGGLRLGPQLTLPPTLSLSRGPPDPPRRPTDEERHKHRRRALGLPKNHLRSDDEGD
  ENKENKPPANEDELEEEAETKQTAVESLLRKLEVAIDQLHDQVYRDLNDFKRRLGIRI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084350
• RefSeq ORF: 384
• Locus ID: 8223421
• MW: 14.7 kDa