E7 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101790

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084347


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol E7 protein
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101790 represents NCBI reference of YP_003084347 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGGAGATCGCGTTACGATCAAGGACGTCGAACTCCATCTCGAGGAGCTTGTGATACCCGCTAACT
TGTTGTCTGACGAGAGCCTTTCTCTCGACGAAACACCCGAGGAGGAGCAACTGTCTCCCTACCGGGTGGA
TTCCCTGTGTACAAGATGCAACAAGTGCATCCGAATCTCAGTCGTTTGTACCACTGGAGCTATTTATACA
CTGGAGCAGCTCTTGTTGAGCACCGAGTTGTCTTTTTTGTGCGCCGGTTGCTCTCGAACCACAGTGAGAA
ATGGACGGAGATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101790 representing YP_003084347
Red=Cloning sites Green=Tags

MKGDRVTIKDVELHLEELVIPANLLSDESLSLDETPEEEQLSPYRVDSLCTRCNKCIRISVVCTTGAIYT
LEQLLLSTELSFLCAGCSRTTVRNGRRF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_013035
ORF Size 294 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_013035.1, YP_003084347
RefSeq ORF 294
Locus ID 8223418
MW 11.0 kDa

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