L1 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101795

Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084352

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101795 represents NCBI reference of YP_003084352 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGTGGACCCCTAACACGGGAAGGCTGTACCTCCCTCCAGCTAAACCCGTGGCAACAGTGCTTA
  GTACCGACGACTATATTGTCCCCACAAACCTGTATTTCCACGCCAACTCTGACCGCCTGTTGACTGTCGG
  TCACCCCTACTTCGACGTTCTGAACGACGCCACCAAGGCTATCGAGGTTCCTAAGGTCTCCGGCAACCAG
  TTTAGAGTACTTAGGCTGAAGTTGCCTGACCCGAATAAGTTTGCCTTGATAGACAAGAGCGTGTACAATC
  CGGAGAAGGAACGCCTGGTGTGGAGATTGAGAGGCGTCCAGATAGATCGCGGTGGCCCCCTGGGAATCGG
  CACTACAGGCCATCCGCTGTTCGATAGGTTGGCAGACACTGAGAATCCCAATGTCTACGCTCCAGCCGAG
  GTGGACGACTCCAGACAGAATATGTCATTCGATCCCAAGCAGAACCAGCTGTTCATCGTCGGGTGCCTCC
  CTGCCACAGGCCAGCATTGGGACATTGCCGAGCCGTGTAAGGACCCGGCTCCCCCTCCCAATAGCTGCCC
  TCCGATTAAACTTATGCACAGCATAATCCAGGATGGTGACATGAGTGACATCGGGCTGGGTAACGTGAAC
  TTTAATAATTTCTCCGCCTCTCGGTCAGACGCTCCCCTCGACGTGATCAATTCAGTGTGTAAATGGCCAG
  ACTTTGTCCAGATGACTAAAGACACCTATGGCGATAGAGTGTGGTTTTTTGGTAAAAGGGAGCAGGTGTA
  TACTCGCCATATGCTGGTTAAGGGGGGAGTCGACGGTGACTCCCTGCCCCACGAGCCCACTAGAGCATAT
  TACATAACACCCAACACCGGAACCCTTCCAGATGGAAATCTCGGCAAGATCAGCTATTTCCCGACGCCTA
  GCGGATCTCTCGTGTCCTCAGAAGCCACAATTTTCAATAGGCCATACTGGCTCCACCAGGCTCAAGGTAA
  GAACAATGGGATTGCTTGGGGGAACAACATATTCATAACTTTGCTGGATAACACTCACAATACTAATTTT
  ATACTGTCCGTCTACACCGGCGCTAGACCAATGGAAGAAGGATATACATACAAGAAGGCTGATTTTAAGA
  AATTCCTGCGGCACACTGAAGAGCTCGAGCTGGAAATTGTCATGCAACTTTGCAAGGTCCCTTTGGAGGC
  TAATGTGCTGGCCCACATTAATGCTATGGATCCAACAATACTCGAGAACTGGCAACTGGCCTTCGTACCG
  GCTCCTCCGCAGAACCTCGAGGACACCTATCGGTATATCCAATCCTTGGCCACAATGTGTCCCGCCGACG
  TGCCCCCCGCTAACAAACCCGACCCTTTTGAGAAGTACTCCTTCTGGGACGTCGACTTGACAGACAAGTT
  TACATCCGAACTCGACCAAACTCCCCTGGGACGCAAGTTCCTCTACACCATGGGAATGCTTAATGGCAGA
  AAGAGACCTCGGGTGGACTACACCACTGGCAACACTACGGTGAAACGGGTCGGAAAGACTGCGAAGAGAA
  GGAAAACTCGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101795 representing YP_003084352
  Red=Cloning sites Green=Tags
  
  MATWTPNTGRLYLPPAKPVATVLSTDDYIVPTNLYFHANSDRLLTVGHPYFDVLNDATKAIEVPKVSGNQ
  FRVLRLKLPDPNKFALIDKSVYNPEKERLVWRLRGVQIDRGGPLGIGTTGHPLFDRLADTENPNVYAPAE
  VDDSRQNMSFDPKQNQLFIVGCLPATGQHWDIAEPCKDPAPPPNSCPPIKLMHSIIQDGDMSDIGLGNVN
  FNNFSASRSDAPLDVINSVCKWPDFVQMTKDTYGDRVWFFGKREQVYTRHMLVKGGVDGDSLPHEPTRAY
  YITPNTGTLPDGNLGKISYFPTPSGSLVSSEATIFNRPYWLHQAQGKNNGIAWGNNIFITLLDNTHNTNF
  ILSVYTGARPMEEGYTYKKADFKKFLRHTEELELEIVMQLCKVPLEANVLAHINAMDPTILENWQLAFVP
  APPQNLEDTYRYIQSLATMCPADVPPANKPDPFEKYSFWDVDLTDKFTSELDQTPLGRKFLYTMGMLNGR
  KRPRVDYTTGNTTVKRVGKTAKRRKTRM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084352
• RefSeq ORF: 1554
• Locus ID: 8223423
• MW: 58.4 kDa