L2 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101794

Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084351

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101794 represents NCBI reference of YP_003084351 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCGGATCAGGAGAAAAAGAGCCTCTCCCACAGATCTTCATAGGAGTTGCGCACTCGGAGGCGATT
  GCTTCCCCGACGTGGAGAATAAGCTGTCAGGCAATACCTTGGCCGACATTCTGCTCAAAGCATTCGGAAG
  CATACTCTACCTTGGAAATCTCGGAATCGGTACAGGAAAGGGTGGGGGGGGTCAGTACGGCTATACTCCC
  TTTGGAGGTACCCGGCCTAGCATCAGTCGCGCACCAGTGAGGCCGGCTATTCCGGTAGATACTGTCGTCC
  CAGGCGAAGTTCTGCCTGTGACACCACTGGATCCAGCCATCGTGCCGCTTACGGACGGTCTTCCTGAGCC
  CGCTGTTATCGACGTACCCGGTGCCGGACCTGGACTGCCTACTGAAACAATCGATGTCACGACCGAACTT
  GACTTGGTGTCTGAAGTGACCGGGGTGGGCGAGCATCCTAGCGTGACATACGATACTAATAACGTGGCAC
  AAATTGACGTGCAGGTTCAACCGCCCCCCCCCAAAAGGATTCTTTTGGACACGAGCATCACAGACACGGA
  ACTCGCCGTACAAACTCATGCATCTCATGTGGATGAGCATTACAATGTGTTTGTGGACGCCCAGTTTCAC
  GGCGAACATATCGGCGCTTTTGAACCAGAATCAATCGAGCTCCAGGAGATCAACCTCCGCCAGGAGTTCG
  AAATTGATGAAGGACCGTTGCGATCAACCCCTCTGTCCTCACGGGCCATTTCCCGCGCCCGAGACCTCTA
  CAACAGATATGTGCAGCAGGTACCAACTACGCAACTCGCAAGTGTCTCCACCCCTAGAGTCACATTTGAA
  TTTGAAAATCCAGCTTTCGAAGCAGAGATCGCGGATGTCTTTAACAGAGAAGTGCAGGAACTGGCGACCC
  AAGGGCAGGACGAGGCGGGCACCGACCTGATCAGACTCAGCGATATTAGGTACGGGGAGTCACCTGCAGG
  GACAGTTCGAGTGTCAAGGCTGGGACAGCGAGAAGGGATGATTATGAGATCCGGACTGCAAGTCGGCCAG
  CGGGTCCACTTTTATTATGACATCTCTCCTATTCCTAAAGAGGCAATTGAGCTGCGAACATTTGGGGAAT
  ATTCCCACGAGTACACTGTGGTAGACGACCTGGCAAGCTCATCTTTTATCAACCCCTTTGAGCAACCAGT
  AGATGGAAGCTTGGAATTCAGCGATGCTGCACTGATCGATTCTGTCGAGGAAGATTTTAGTGGCACCCAC
  CTGATTCTGACATCAGCAAACGCAGCAGACTCAATTGATATTCCTGTTATTCCACCAGGAATCGGCGTGC
  GAGTGTTCGTGGACGACTATGCAAAAGGGCTCTCAGTACTGCACCCTACAATAATCGATAACGGGGCCAT
  ATATCCAACTGACATTTCATCCAACATCCTCCCCTTGACCCCATCATTTTCAATTGACGTCAACTACAGC
  GATTATAACATCCACCCCGCTAACATACGGAGGAAGAGGAAACATTCCTCCAGTCTCTATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101794 representing YP_003084351
  Red=Cloning sites Green=Tags
  
  MMRIRRKRASPTDLHRSCALGGDCFPDVENKLSGNTLADILLKAFGSILYLGNLGIGTGKGGGGQYGYTP
  FGGTRPSISRAPVRPAIPVDTVVPGEVLPVTPLDPAIVPLTDGLPEPAVIDVPGAGPGLPTETIDVTTEL
  DLVSEVTGVGEHPSVTYDTNNVAQIDVQVQPPPPKRILLDTSITDTELAVQTHASHVDEHYNVFVDAQFH
  GEHIGAFEPESIELQEINLRQEFEIDEGPLRSTPLSSRAISRARDLYNRYVQQVPTTQLASVSTPRVTFE
  FENPAFEAEIADVFNREVQELATQGQDEAGTDLIRLSDIRYGESPAGTVRVSRLGQREGMIMRSGLQVGQ
  RVHFYYDISPIPKEAIELRTFGEYSHEYTVVDDLASSSFINPFEQPVDGSLEFSDAALIDSVEEDFSGTH
  LILTSANAADSIDIPVIPPGIGVRVFVDDYAKGLSVLHPTIIDNGAIYPTDISSNILPLTPSFSIDVNYS
  DYNIHPANIRRKRKHSSSLYF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084351
• RefSeq ORF: 1533
• Locus ID: 8223422
• MW: 55.7 kDa