E1 protein (NC_013035) Virus Tagged ORF Clone

CAT#: VC101791

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 116], codon optimized for human cell expression, YP_003084348

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1 protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101791 represents NCBI reference of YP_003084348 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACGCTTCAAAAGGTATAGATTCCGTCGACGATTCATCATGGTTTATAGTGAACGAGGCGGACT
  GTATGGACGATATAGAAACCCTGGACACCCTCTTTGATGAGAGTGACTGCGACTCCACAGTTTCCAACCT
  TATTGACGACGAGAACTTTCAAGAACAAGGCAACTCTCTCGCCCTGTTCAACATTCAGTGCGCCGAGGAG
  TGCGACAAAACGGTAAGCCTCTTGAAGAGAAAATATGCCCAGTCCCCACAGGGCTCCAGCGTGGCTGAAC
  TCAGTCCGAGACTGGAGGCCGTGAAGATTTCCCCAGAAAAGGAACGCCAATCAAAAAGGCGGCTGTTCCA
  GGACAGCGGACTGGGAGAGGACGAGGCCGAGCTGATCTCCGAGCAGGTTGAGGTTCAGACTAACGAGAAT
  GGTGGGGATACCTTGTCTGCAGCATGTAACAGCATTCTGAATAGCAAATGTAAGAGGTCTCTCCTGTTTG
  TGAAATGTGAGACATTGTTCGGCGTCAGTTATAATGAGCTTACAAGACAGTTTAAATCCCACAAGAGCTG
  CTGCGAAAACTGGATTGTCTTCGTCTATGCTGCGGGAACTGAAGTGCTCGAGAGTTCCAAGGTGCTGCTG
  CAACAGCATTGCGAGAATTTTCAGGTGATTTTGTGTGATTTTTCCGGGCTGTACGTTCTGCAATTCAAGC
  ACGGCAAAAATAGAGAAACTGTGGAAAGACTGTTTTGCAATATACTTCATGTGACAGATTCACACTTGCT
  GTCCGATCCCCCCCGGAGCCGCAGTGTCCCTGCGGCTCTGTTCTTTTTTAAGCGAAGCGTGAGCAAGACC
  AGTTATGTCTACAATAACTTGCCTGCTTGGGTCACAAAGCTCACGCAGTTTAACCACCAAGTGGCCACAC
  AGCCTGAAGCATTTGAGCTCTCTAAAATGATCCAGTGGGCGTATGATAACAGGATGACTGAGGAGGCCGA
  GATCGCCTACGGCTACGCACTGCTCGCAGACGAGGACACTAACGCCGCCGCCTTCTTGAAAAGCAACGTA
  CAGCTGAAATATGTGCGGGACTGCTGCACCATGGTCAAGCTCTACTTTAGACAGGAAATGCGGGAGATGA
  GCATTAGCCAGTGGATTTGGAAATGTTGCAATGACTGCGAGGGCGAGGCCGACTGGAAGCTCATTCTGAA
  TTTCTTGAAATTTCAAAACATTAACGTGATTCAATTCCTGACCTGCCTGAGAACACTCTGCAAACGCATT
  CCAAAGAAGAACTGTATTTTGTTTTACGGTCCTCCAGATACCGGCAAATCTTTTTTCGCCTACAGCCTGG
  TGAAGTTCCTGCAGGGGAAAATTCTGAGCTTTGTCAACAAGACCAGCAATTTTTGGCTTCAGCCTCTGCT
  GGATTGCAAGTTCGCTCTCCTTGACGACGTGACTTACCCCTGTTGGCAATACATTGACCAAAATATGCGC
  GGCGCCCTCGACGGCAATACAATGTGCGTTGATGCTAAACACAGAGTGCCTCAACAGATTAAGTTGCCCC
  CACTCATTTTGACCTCCAACATTGATGTGGTGAAAGAAGAGAGCCTTCAGTACCTGCATTCTCGACTCAT
  GTGTTTCGAATTTGCTAACAAGTTGCCTTTCGATACCCACGGCATTCCCGTCTACAATTTCACAAACCAG
  GTTTGGAAGTGCTTTTTCCAGAAGCTGTCTCGCCAACTCGACCTGGAGGAGGAAAATATACAACATGAAT
  CAATAAGGTCAGACCGAACATTTCGATGTATCGCCAATTCACCCGATGAGCATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101791 representing YP_003084348
  Red=Cloning sites Green=Tags
  
  MADASKGIDSVDDSSWFIVNEADCMDDIETLDTLFDESDCDSTVSNLIDDENFQEQGNSLALFNIQCAEE
  CDKTVSLLKRKYAQSPQGSSVAELSPRLEAVKISPEKERQSKRRLFQDSGLGEDEAELISEQVEVQTNEN
  GGDTLSAACNSILNSKCKRSLLFVKCETLFGVSYNELTRQFKSHKSCCENWIVFVYAAGTEVLESSKVLL
  QQHCENFQVILCDFSGLYVLQFKHGKNRETVERLFCNILHVTDSHLLSDPPRSRSVPAALFFFKRSVSKT
  SYVYNNLPAWVTKLTQFNHQVATQPEAFELSKMIQWAYDNRMTEEAEIAYGYALLADEDTNAAAFLKSNV
  QLKYVRDCCTMVKLYFRQEMREMSISQWIWKCCNDCEGEADWKLILNFLKFQNINVIQFLTCLRTLCKRI
  PKKNCILFYGPPDTGKSFFAYSLVKFLQGKILSFVNKTSNFWLQPLLDCKFALLDDVTYPCWQYIDQNMR
  GALDGNTMCVDAKHRVPQQIKLPPLILTSNIDVVKEESLQYLHSRLMCFEFANKLPFDTHGIPVYNFTNQ
  VWKCFFQKLSRQLDLEEENIQHESIRSDRTFRCIANSPDEHL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_013035
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_013035.1, YP_003084348
• RefSeq ORF: 1806
• Locus ID: 8223419
• MW: 69.2 kDa