N (NC_003461) Virus Tagged ORF Clone

CAT#: VC102042

Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604433

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102042 represents NCBI reference of NP_604433 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTCTCCTGTCCACCTTTGATACCTTCTCTAGTCGCCGAAGCGAGTCCATCAACAAGAGCGGTG
  GAGGTGCCATCATCCCTGGCCAGCGCTCCACTGTGAGTGTGTTCACCCTCGGACCTTCAGTGACTGACGA
  TGCGGATAAGCTGTTGATCGCAACAACATTCCTGGCACACTCTCTTGATACCGATAAGCAGCACAGCCAA
  CGGGGCGGTTTTCTGGTCTCTCTGCTGGCCATGGCGTATAGCTCACCTGAGCTGTACCTCACTACTAATG
  GCGTCAATGCGGACGTCAAGTACGTAATCTATAATATTGAGAGAGACCCTAAACGCACTAAAACAGACGG
  ATTTATAGTCAAGACCAGAGATATGGAGTATGAGAGGACTACAGAGTGGCTCTTTGGCCCAATGATCAAC
  AAGAACCCTCTCTTCCAGGGCCAGCGGGAGAACGCCGATCTTGAAGCACTTCTGCAGACTTACGGATATC
  CTGCATGCCTCGGGGCCATTATTGTTCAAGTGTGGATCGTGCTGGTTAAGGCTATCACATCAAGCGCGGG
  GCTGAGGAAGGGTTTTTTTAATAGGCTGGAAGCGTTCAGACAGGACGGAACTGTAAAATCAGCTCTGGTC
  TTCACCGGAGATACCGTGGAAGGAATTGGGGCTGTAATGCGCAGCCAGCAGTCCTTGGTGAGTCTCATGG
  TGGAAACCCTGGTCACCATGAATACAAGCCGATCTGACTTGACCACCCTTGAGAAGAATATTCAGATCGT
  GGGCAATTACATCCGGGACGCCGGGCTGGCAAGTTTTATGAATACAATCAAGTACGGGGTCGAGACCAAA
  ATGGCTGCTCTGACCCTGAGTAACCTCAGGCCTGATATCAACAAACTGAGGTCCCTCGTAGACATTTATC
  TGTCAAAGGGCGCTCGGGCTCCATTCATCTGCATCCTCAGAGACCCAGTTCATGGAGACTTCGCCCCCGG
  TAACTATCCCGCGCTTTGGTCCTATGCTATGGGAGTTGCTGTTGTCCAGAACAAAGCTATGCAGCAATAT
  GTAACTGGCCGCACGTACCTTGATATGGAGATGTTCCTGCTGGGGCAGGCCGTGGCGAAAGATGCCGACT
  CCAAGATTAGTTCAGCTCTTGAAGAAGAGCTGGGCGTGACTGATACTGCCAAGGAGCGGCTGCGCCATCA
  CCTTACAAACCTCTCCGGTGGCGATGGCGCCTACCACAAGCCCACAGGCGGAGGGGCAATCGAAGTGGCC
  ATCGACCATACAGATATTACCTTCGGAGTTGAGGATACTGCAGATAGAGACAATAAGAACTGGACTAATG
  ATTCTAATGAAAGATGGATGAACCATAGCATAAGCAACCATACTATCACTATCCGGGGAGCTGAGGAGCT
  GGAAGAAGAAACCAACGATGAGGACATAACAGATATTGAGAATAAAATCGCTCGAAGATTGGCAGATAGA
  AAACAGCGGCTTTCTCAGGCTAACAACAAGCGGGATACTAGCAGCGATGCCGATTACGAGAACGACGATG
  ATGCGACCGCTGCCGCTGGAATCGGGGGAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102042 representing NP_604433
  Red=Cloning sites Green=Tags
  
  MAGLLSTFDTFSSRRSESINKSGGGAIIPGQRSTVSVFTLGPSVTDDADKLLIATTFLAHSLDTDKQHSQ
  RGGFLVSLLAMAYSSPELYLTTNGVNADVKYVIYNIERDPKRTKTDGFIVKTRDMEYERTTEWLFGPMIN
  KNPLFQGQRENADLEALLQTYGYPACLGAIIVQVWIVLVKAITSSAGLRKGFFNRLEAFRQDGTVKSALV
  FTGDTVEGIGAVMRSQQSLVSLMVETLVTMNTSRSDLTTLEKNIQIVGNYIRDAGLASFMNTIKYGVETK
  MAALTLSNLRPDINKLRSLVDIYLSKGARAPFICILRDPVHGDFAPGNYPALWSYAMGVAVVQNKAMQQY
  VTGRTYLDMEMFLLGQAVAKDADSKISSALEEELGVTDTAKERLRHHLTNLSGGDGAYHKPTGGGAIEVA
  IDHTDITFGVEDTADRDNKNWTNDSNERWMNHSISNHTITIRGAEELEEETNDEDITDIENKIARRLADR
  KQRLSQANNKRDTSSDADYENDDDATAAAGIGGI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604433
• RefSeq ORF: 1572
• Locus ID: 935258
• MW: 57.6 kDa