C' (NC_003461) Virus Tagged ORF Clone

CAT#: VC102043

Myc-DDK-tagged ORF clone of viral ORF for C' protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604434

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C'
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102043 represents NCBI reference of NP_604434 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACTAGCGCCTCCAAGACCCTCCTTCCAGAGTGGATACGCATGCCTAGCTTTCTCAGAGGGATCC
  TCAAACCCAAGGAGCGCCATCACGAAAATAAGAACCACTCACAGATGTCCTCAGATTCACTGACGTCCAG
  CTACCCTACCTCTCCTCAGAAACTGGAGAAGACTGAGGCCGGGAGCATGGTTAGCTCCACCACCCAGAAG
  AAGACATCACACCACGCCAAGCCCACTATTACCACCAAGACAGAACAATCTCAGCGCCGCCCGAAAATTA
  TAGATCAGGTGCGGGGGGTAGAATCACTGGGTGAACAAGTTTCCCAGAAGCAGAGGCACATGCTCGAGAG
  CCTCATTAACAAAGTATACACCGGTCCTCTGGGGGAGGAGCTGGTCCAAACCCTTTACCTCAGAATATGG
  GCTATGAAGGAGACCCCAGAAAGTACTAAAATCCTCCAAATGCGCGAAGATATTAGGGATCAGTACCTGC
  GGATGAAGACAGAGCGCTGGCTTCGAACTCTGATAAGGGGGAAAAAGACAAAGCTCAGGGATTTTCAGAA
  GCGCTATGAGGAAGTGCATCCCTACCTGATGATGGAGCGCGTGGAACAGATAATTATGGAAGAGGCCTGG
  AAACTCGCTGCCCACATTGTCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102043 representing NP_604434
  Red=Cloning sites Green=Tags
  
  MDTSASKTLLPEWIRMPSFLRGILKPKERHHENKNHSQMSSDSLTSSYPTSPQKLEKTEAGSMVSSTTQK
  KTSHHAKPTITTKTEQSQRRPKIIDQVRGVESLGEQVSQKQRHMLESLINKVYTGPLGEELVQTLYLRIW
  AMKETPESTKILQMREDIRDQYLRMKTERWLRTLIRGKKTKLRDFQKRYEEVHPYLMMERVEQIIMEEAW
  KLAAHIVQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604434
• RefSeq ORF: 657
• Locus ID: 935259
• MW: 25.8 kDa