M (NC_003461) Virus Tagged ORF Clone

CAT#: VC102048

Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604439

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102048 represents NCBI reference of NP_604439 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGACTTATAGGTTCCCCCGCTTTTCCCATGAGGAGAACGGTACGGTCGAGCCTCTGCCACTCA
  AGACCGGCCCCGATAAGAAGGCGATTCCTCATATTAGGATTGTTAAAGTAGGTGACCCACCAAAACACGG
  TGTCCGGTATCTTGATGTGCTGCTTCTCGGCTTCTTCGAAACGCCCAAGCAGGGTCCACTCTCTGGTAGC
  ATCTCAGACCTCACCGAGTCCACCAGCTACTCTATTTGCGGGTCAGGGTCATTGCCTATAGGGATTGCAA
  AATACTATGGCACCGACCAGGAATTGCTTAAAGCTTGTATCGATCTCAAAATTACTGTTCGGCGCACAGT
  GAGATCCGGCGAGATGATTGTCTATATGGTGGATTCAATACACGCGCCATTGCTGCCCTGGAGCTCCCGG
  CTCCGACAGGGCATGATCTACAATGCCAATAAAGTTGCTCTGGCACCCCAGTGTCTGCCTGTCGATAAGG
  ACATCCGCTTTCGGGTCGTGTTTGTTAACGGGACTTCTCTGGGCACCATAACCATTGCGAAGGTCCCTAA
  GACGCTTGCCGATCTCGCCCTCCCAAACAGCATTAGTGTTAATCTGCTCGTTACCTTGAGAGCCGGCGTA
  TCCACTGAACAGAAGGGCATCCTGCCTGTGCTGGACGATGACGGGGAGAAGAAACTTAATTTCATGGTCC
  ACCTTGGCATTATAAGGAGAAAAGTCGGAAAGATTTACTCTGTGGAATACTGTAAAAACAAGATCGAAAA
  AATGAAGCTGATCTTCAGTCTGGGCCTGGTAGGCGGTATTTCATTCCACGTCCACGCTACCGGGACACTC
  TCCAAGACGCTGATGAGCCAACTGGCTTGGAAGAAAGCCGTGTGTTACCCTCTCATGGACGTAAACCCAC
  ATATGAATCTCGTGATTTGGGCAGCCTCCGTTGAAATCACTTCAGTCGACGCCGTGTTCCAGCCTGCCAT
  CCCTAAAGAGTTTCGATATTACCCCAACGTCGTTGCCAAATCCATCGGGAAGATTAGGCGCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102048 representing NP_604439
  Red=Cloning sites Green=Tags
  
  MAETYRFPRFSHEENGTVEPLPLKTGPDKKAIPHIRIVKVGDPPKHGVRYLDVLLLGFFETPKQGPLSGS
  ISDLTESTSYSICGSGSLPIGIAKYYGTDQELLKACIDLKITVRRTVRSGEMIVYMVDSIHAPLLPWSSR
  LRQGMIYNANKVALAPQCLPVDKDIRFRVVFVNGTSLGTITIAKVPKTLADLALPNSISVNLLVTLRAGV
  STEQKGILPVLDDDGEKKLNFMVHLGIIRRKVGKIYSVEYCKNKIEKMKLIFSLGLVGGISFHVHATGTL
  SKTLMSQLAWKKAVCYPLMDVNPHMNLVIWAASVEITSVDAVFQPAIPKEFRYYPNVVAKSIGKIRRI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604439
• RefSeq ORF: 1044
• Locus ID: 935264
• MW: 38.5 kDa