HN (NC_003461) Virus Tagged ORF Clone

CAT#: VC102050

Myc-DDK-tagged ORF clone of viral ORF for HN glycoprotein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604441

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102050 represents NCBI reference of NP_604441 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAAAGGGTAAGACAAATTCTTCATATTGGTCCACCACTAGAAACGATAATTCAACCGTGAATA
  CTCATATAAACACCCCAGCAGGTAGGACGCATATCTGGCTCTTGATCGCTACGACGATGCACACAGTGCT
  TTCATTTATTATCATGATCCTTTGTATCGATCTGATCATCAAGCAGGATACTTGTATGAAAACGAATATT
  ATGACCGTCTCCAGCATGAACGAATCCGCCAAGATTATAAAGGAGACCATCACAGAACTTATTCGCCAGG
  AAGTGATCTCACGAACTATCAACATCCAGTCCTCTGTCCAGTCAGGCATCCCGATCTTGCTGAATAAACA
  GTCCAGAGACCTGACCCAGCTGATTGAAAAAAGCTGCAACAGACAGGAGCTGGCTCAGATCTGCGAGAAT
  ACAATTGCAATTCACCATGCCGACGGAATTTCACCACTCGATCCCCACGACTTTTGGCGGTGTCCTGTCG
  GAGAACCACTGCTCTCTAATAATCCTAACATCTCACTGCTGCCTGGTCCCTCTCTTCTGTCAGGGTCAAC
  CACCATCTCTGGATGCGTCCGCTTGCCTTCACTTTCAATAGGAGATGCGATCTATGCTTATAGCTCAAAT
  CTGATTACTCAGGGTTGTGCAGATATTGGGAAGTCTTACCAAGTGCTGCAACTCGGATATATCTCACTTA
  ACTCTGATATGTATCCTGATCTTAATCCAGTTATTAGTCACACCTATGACATTAACGATAACAGAAAGAG
  CTGTTCTGTGATTGCAGCAGGGACTCGCGGGTACCAACTCTGTAGCCTGCCTACTGTGAACGAAACCACC
  GATTACTCCAGCGAGGGTATCGAAGACCTGGTGTTCGACATCCTGGATTTGAAAGGAAAAACCAAAAGTC
  ACAGGTATAAGAATGAAGATATCACCTTCGATCACCCTTTCTCAGCAATGTATCCTTCAGTGGGGTCTGG
  CATCAAAATCGAGAACACCCTCATATTCCTTGGGTATGGAGGGCTGACCACACCCCTGCAGGGGGATACC
  AAGTGTGTGATTAATAGGTGCACCAACGTTAACCAGTCCGTTTGCAATGACGCCCTGAAAATCACATGGT
  TGAAAAAAAGGCAGGTAGTGAACGTCCTCATCAGGATTAATAACTACCTGTCAGATAGGCCAAAAATTGT
  TGTGGAAACCATACCAATTACTCAGAACTACCTCGGCGCTGAGGGCCGGCTCCTCAAGCTGGGTAAAAAA
  ATATACATCTATACTCGGAGCTCCGGCTGGCACTCAAATCTCCAGATAGGCTCCTTGGACATCAACAATC
  CTATGACCATCAAGTGGGCACCGCACGAGGTCCTGTCACGCCCAGGAAATCAAGATTGTAATTGGTATAA
  TAGATGCCCACGCGAATGTATCAGCGGGGTCTATACTGATGCGTACCCCCTGAGCCCAGACGCTGTAAAT
  GTTGCCACGACCACCCTTTACGCAAATACAAGCCGCGTGAACCCAACTATTATGTACAGCAACACCAGCG
  AAATCATTAACATGCTGAGACTTAAAAACGTGCAGCTCGAGGCTGCATATACGACAACATCATGCATCAC
  ACACTTTGGAAAGGGCTACTGCTTTCACATCGTCGAGATTAATCAGGCAAGCCTCAACACACTGCAGCCC
  ATGCTCTTTAAGACCTCTATCCCAAAAATCTGTAAAATTACCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102050 representing NP_604441
  Red=Cloning sites Green=Tags
  
  MAEKGKTNSSYWSTTRNDNSTVNTHINTPAGRTHIWLLIATTMHTVLSFIIMILCIDLIIKQDTCMKTNI
  MTVSSMNESAKIIKETITELIRQEVISRTINIQSSVQSGIPILLNKQSRDLTQLIEKSCNRQELAQICEN
  TIAIHHADGISPLDPHDFWRCPVGEPLLSNNPNISLLPGPSLLSGSTTISGCVRLPSLSIGDAIYAYSSN
  LITQGCADIGKSYQVLQLGYISLNSDMYPDLNPVISHTYDINDNRKSCSVIAAGTRGYQLCSLPTVNETT
  DYSSEGIEDLVFDILDLKGKTKSHRYKNEDITFDHPFSAMYPSVGSGIKIENTLIFLGYGGLTTPLQGDT
  KCVINRCTNVNQSVCNDALKITWLKKRQVVNVLIRINNYLSDRPKIVVETIPITQNYLGAEGRLLKLGKK
  IYIYTRSSGWHSNLQIGSLDINNPMTIKWAPHEVLSRPGNQDCNWYNRCPRECISGVYTDAYPLSPDAVN
  VATTTLYANTSRVNPTIMYSNTSEIINMLRLKNVQLEAAYTTTSCITHFGKGYCFHIVEINQASLNTLQP
  MLFKTSIPKICKITS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604441
• RefSeq ORF: 1725
• Locus ID: 935266
• MW: 64.0 kDa