Y1 (NC_003461) Virus Tagged ORF Clone

CAT#: VC102046

Myc-DDK-tagged ORF clone of viral ORF for Y1 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604437

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Y1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102046 represents NCBI reference of NP_604437 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCGACAGCCTTACCTCATCTTATCCTACTAGCCCTCAGAAATTGGAGAAGACCGAGGCCGGTT
  CTATGGTCAGCTCCACTACTCAGAAGAAGACCTCCCATCATGCTAAGCCAACGATTACCACTAAGACCGA
  ACAAAGCCAGAGGAGGCCCAAAATCATCGACCAAGTCCGGGGGGTAGAATCCCTCGGAGAGCAGGTCAGC
  CAGAAGCAGAGGCATATGCTTGAGTCTCTGATTAACAAGGTCTACACCGGGCCACTGGGAGAGGAACTCG
  TGCAGACACTTTATCTGAGAATTTGGGCTATGAAGGAAACCCCCGAGTCAACAAAAATTCTTCAGATGCG
  GGAAGATATAAGAGACCAGTACTTGCGCATGAAAACCGAGAGATGGCTGCGGACCCTTATCCGGGGCAAA
  AAGACTAAATTGCGGGATTTCCAAAAACGATATGAGGAGGTTCACCCCTACCTGATGATGGAGAGAGTGG
  AGCAGATCATCATGGAAGAGGCCTGGAAACTCGCAGCCCACATTGTGCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102046 representing NP_604437
  Red=Cloning sites Green=Tags
  
  MSSDSLTSSYPTSPQKLEKTEAGSMVSSTTQKKTSHHAKPTITTKTEQSQRRPKIIDQVRGVESLGEQVS
  QKQRHMLESLINKVYTGPLGEELVQTLYLRIWAMKETPESTKILQMREDIRDQYLRMKTERWLRTLIRGK
  KTKLRDFQKRYEEVHPYLMMERVEQIIMEEAWKLAAHIVQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 543 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604437
• RefSeq ORF: 543
• Locus ID: 935262
• MW: 21.3 kDa