Y2 (NC_003461) Virus Tagged ORF Clone

CAT#: VC102047

Myc-DDK-tagged ORF clone of viral ORF for putative Y2 protein [Human parainfluenza virus 1], codon optimized for human cell expression, NP_604438

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Y2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102047 represents NCBI reference of NP_604438 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCTATCCTACATCCCCCCAGAAACTGGAGAAGACCGAAGCTGGCTCTATGGTTAGTAGTACCA
  CTCAGAAAAAAACTAGCCACCACGCAAAGCCAACCATAACAACTAAAACTGAGCAGAGCCAGAGGCGGCC
  AAAAATCATTGATCAAGTTAGAGGAGTTGAATCCCTTGGGGAACAGGTGTCCCAGAAACAGAGGCACATG
  CTGGAAAGCTTGATCAACAAGGTCTACACCGGCCCACTGGGCGAGGAACTGGTGCAGACCCTGTACCTCA
  GAATTTGGGCCATGAAGGAAACCCCTGAGTCTACCAAAATCCTCCAAATGCGAGAAGATATCAGGGACCA
  GTACCTGCGCATGAAAACTGAGAGATGGTTGCGCACCCTCATACGCGGTAAGAAAACTAAACTGAGGGAC
  TTTCAGAAGCGGTACGAGGAGGTTCATCCTTACCTGATGATGGAGAGGGTGGAGCAGATAATTATGGAAG
  AAGCCTGGAAGCTGGCCGCCCACATTGTGCAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102047 representing NP_604438
  Red=Cloning sites Green=Tags
  
  MSSYPTSPQKLEKTEAGSMVSSTTQKKTSHHAKPTITTKTEQSQRRPKIIDQVRGVESLGEQVSQKQRHM
  LESLINKVYTGPLGEELVQTLYLRIWAMKETPESTKILQMREDIRDQYLRMKTERWLRTLIRGKKTKLRD
  FQKRYEEVHPYLMMERVEQIIMEEAWKLAAHIVQE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003461
• ORF Size: 525 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_003461.1, NP_604438
• RefSeq ORF: 525
• Locus ID: 935263
• MW: 20.7 kDa