nonstructural protein NS3 (NC_012532) Virus Tagged ORF Clone

CAT#: VC102534

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227202.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS3
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102534 represents NCBI reference of YP_009227202 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCGCACTGTGGGACGTGCCCGCCCCAAAGGAAGTCAAGAAGGGGGAAACTACCGATGGCGTAT
  ATAGAGTTATGACAAGAAGACTGCTGGGCTCCACACAGGTAGGAGTGGGCGTGATGCAGGAAGGAGTTTT
  TCATACTATGTGGCACGTGACTAAGGGCGCAGCACTGCGAAGTGGAGAGGGAAGGCTTGATCCTTATTGG
  GGCGATGTGAAACAGGACCTGGTTTCATACTGCGGGCCGTGGAAGTTGGATGCTGCGTGGGACGGCCTGT
  CTGAAGTTCAGCTCCTTGCTGTGCCTCCCGGAGAACGAGCTAGGAATATTCAAACTCTGCCAGGTATCTT
  TAAGACAAAGGACGGAGACATTGGAGCCGTTGCCCTTGACTACCCAGCCGGCACCAGCGGTAGTCCAATC
  CTCGACAAGTGCGGCCGGGTCATCGGACTTTATGGCAATGGAGTGGTGATTAAAAATGGATCATACGTGA
  GCGCTATAACACAAGGAAAGAGAGAAGAAGAAACCCCAGTTGAATGCTTTGAACCGAGCATGCTCAAGAA
  GAAACAGCTTACCGTGCTTGATTTGCATCCAGGCGCTGGGAAGACTCGCCGCGTTCTCCCTGAAATCGTT
  AGAGAAGCCATCAAGAAGCGCTTGAGAACTGTCATCCTTGCACCCACAAGAGTCGTCGCCGCCGAAATGG
  AAGAGGCTCTCCGAGGTCTTCCAGTCCGGTACATGACAACCGCCGTTAACGTGACACACTCTGGAACCGA
  AATTGTTGACCTTATGTGCCACGCCACGTTCACTTCCAGACTTCTCCAACCTATCAGGGTGCCAAACTAC
  AATCTTAACATCATGGACGAAGCACATTTTACTGACCCCTCAAGTATCGCGGCCCGGGGTTATATTAGCA
  CAAGAGTCGAGATGGGAGAAGCCGCCGCAATCTTTATGACCGCCACTCCACCAGGAACCCGGGATGCCTT
  CCCCGATAGCAACTCACCCATCATGGACACAGAGGTCGAAGTGCCTGAGCGCGCCTGGTCTTCAGGATTC
  GACTGGGTCACTGACCACTCCGGGAAAACCGTATGGTTCGTACCATCCGTTAGAAATGGCAACGAGATTG
  CAGCTTGTCTCACTAAGGCTGGCAAGAGGGTGATCCAGCTTTCCCGAAAGACCTTCGAGACTGAATTTCA
  GAAGACCAAAAACCAGGAATGGGACTTCGTGATTACTACCGATATCAGCGAGATGGGGGCCAACTTTAAA
  GCTGACAGAGTGATCGACAGCAGAAGGTGCCTGAAGCCCGTAATATTGGACGGCGAACGAGTCATTTTGG
  CAGGCCCAATGCCCGTGACCCATGCTTCAGCTGCACAGAGGCGGGGCAGGATCGGCCGGAACCCCAATAA
  GCCCGGTGACGAGTACATGTATGGAGGCGGATGCGCTGAAACTGACGAAGGACATGCACACTGGCTGGAA
  GCCAGAATGCTCCTGGATAATATATATCTCCAAGATGGTCTCATTGCAAGTCTTTACCGCCCTGAGGCTG
  ATAAGGTTGCTGCCATCGAAGGCGAGTTTAAGCTGAGGACAGAGCAAAGGAAAACTTTCGTGGAACTGAT
  GAAGAGAGGTGACCTGCCCGTGTGGCTGGCATACCAGGTGGCTAGTGCCGGAATTACCTACACAGACCGG
  AGATGGTGCTTTGATGGGACCACTAATAACACAATCATGGAAGATAGCGTGCCTGCCGAGGTCTGGACGA
  AGTACGGCGAGAAAAGAGTACTGAAACCCCGCTGGATGGATGCAAGAGTCTGTAGTGACCACGCCGCCCT
  GAAGAGCTTTAAAGAATTTGCGGCTGGGAAACGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102534 representing YP_009227202
  Red=Cloning sites Green=Tags
  
  MSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMWHVTKGAALRSGEGRLDPYW
  GDVKQDLVSYCGPWKLDAAWDGLSEVQLLAVPPGERARNIQTLPGIFKTKDGDIGAVALDYPAGTSGSPI
  LDKCGRVIGLYGNGVVIKNGSYVSAITQGKREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIV
  REAIKKRLRTVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNY
  NLNIMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGF
  DWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKTKNQEWDFVITTDISEMGANFK
  ADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPNKPGDEYMYGGGCAETDEGHAHWLE
  ARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDR
  RWCFDGTTNNTIMEDSVPAEVWTKYGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_012532.1, YP_009227202
• RefSeq ORF: 1854 bp
• MW: 68.5 kDa