nonstructural protein NS2A (NC_012532) Virus Tagged ORF Clone

CAT#: VC102532

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Zika virus, strain MR766], codon optimized for human cell expression, YP_009227200.1

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS2A
• Synonyms: ZIKV_gp1, ZIKV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102532 represents NCBI reference of YP_009227200 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCAACTGATCATATGGACCATTTCTCACTGGGCGTCTTGGTCATCCTGCTGATGGTACAGGAAG
  GATTGAAAAAAAGAATGACTACTAAAATCATTATGTCTACTTCAATGGCTGTTCTTGTTGTTATGATACT
  GGGGGGTTTTTCCATGTCCGATCTTGCTAAGTTGGTAATCTTGATGGGCGCCACATTTGCTGAAATGAAC
  ACTGGGGGTGACGTGGCCCACCTCGCACTCGTCGCTGCTTTTAAAGTTCGCCCCGCCCTGCTCGTAAGCT
  TCATCTTCAGAGCTAACTGGACTCCTAGAGAATCTATGCTTCTCGCTCTCGCCTCATGCCTCCTGCAAAC
  CGCAATTTCTGCCTTGGAAGGAGATCTCATGGTACTTATTAACGGATTTGCCCTCGCCTGGCTTGCTATC
  CGCGCGATGGCCGTTCCAAGAACCGATAATATTGCCCTCCCAATCCTCGCCGCCCTTACTCCCCTTGCAC
  GGGGTACACTGCTGGTAGCCTGGAGAGCTGGCCTCGCCACTTGTGGGGGAATCATGCTGCTGTCACTGAA
  AGGCAAAGGGTCAGTGAAAAAAAATCTGCCATTTGTCATGGCACTTGGACTCACCGCTGTGCGAGTTGTT
  GATCCTATCAATGTTGTGGGACTTCTCCTGCTCACGCGATCAGGTAAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102532 representing YP_009227200
  Red=Cloning sites Green=Tags
  
  MGSTDHMDHFSLGVLVILLMVQEGLKKRMTTKIIMSTSMAVLVVMILGGFSMSDLAKLVILMGATFAEMN
  TGGDVAHLALVAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQTAISALEGDLMVLINGFALAWLAI
  RAMAVPRTDNIALPILAALTPLARGTLLVAWRAGLATCGGIMLLSLKGKGSVKKNLPFVMALGLTAVRVV
  DPINVVGLLLLTRSGKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012532
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_012532.1, YP_009227200
• RefSeq ORF: 681 bp
• MW: 24.1 kDa