Secondary Antibodies

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Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE
Immunogen Goats were hyperimmunized with Mouse IgM, IgG and IgA.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE
Immunogen Goats were hyperimmunized with Mouse IgM, IgG and IgA.

Turkey IgG (H+L chain) goat polyclonal antibody, PE

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry. 
Immunofluorescence: 
≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Conjugation PE
Immunogen Human IgG F(c) fragment.

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG F(ab')2 fragment.

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/200) and Flow Cytometry (1/100-1/200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE
Immunogen Goat IgG whole molecule

Rabbit IgG (H+L chain) sheep polyclonal antibody, PE

Applications Flow Cytometry (Use 50 µl of 1/5-1/10 diluted antibody to label 10e6 cells in 100 µl).
Conjugation PE
Immunogen Highly purified Rabbit IgG

Mouse IgG1 goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG1 paraproteins

Mouse IgG2a goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG2a paraproteins

Mouse IgG2b goat polyclonal antibody, PE

Applications Flow Cytometry: Use 10 µl of 1/20-1/30 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE
Immunogen Mouse IgG2b paraproteins.

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Recommended dilutions:
Flow cytometry: 1:100 - 1:200
IF Mocroscopy: 1:100 - 1:200
Conjugation PE
Immunogen Human IgG whole molecule

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in Flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Conjugation PE
Immunogen Human IgG F(ab')2 fragment.

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 mg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Conjugation PE
Immunogen Mouse IgG whole molecule.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Recommended Dilutions
Immunofluorescence: 1/100-1/250.
Flow Cytometry: 1/100-1/250.
Reactivities Mouse
Conjugation PE
Immunogen Human IgG F(c) fragment.

Rat IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Conjugation PE
Immunogen Rat IgG whole molecule

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:250) and Flow cytometry (1:100-1:250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation PE
Immunogen Rat IgG F(c) fragment

Human IgG (adsorbed F(ab)2 Fragment) donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE
Immunogen Human IgG whole molecule.

Mouse IgM (Mu chain specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.
Recommended dilutions:
Flow cytometry 1:100 - 1:250
IF microscopy 1:100 - 1:250
Conjugation PE
Immunogen Mouse IgM mu heavy chain

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE
Immunogen Rabbit IgG whole molecule.

Rat IgG (H+L chain) F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation PE
Immunogen Rat IgG whole molecule

Rat IgM (Mu chain specific) goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE
Immunogen Rat IgM mu heavy chain.

Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE
Immunogen Sheep IgG whole molecule

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/20 diluted anti body to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation PE
Immunogen Purified mouse IgG

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE
Immunogen Rat IgG