Secondary Antibodies

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Rat IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1:10000.
Western Blot: 1:1000 - 1:10000.
Reactivities Rat
Conjugation HRP

Rat IgM goat polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Reactivities Rat
Conjugation HRP

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP
Immunogen Whole Mouse IgG

Mouse IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/1000-1/10000.
Reactivities Mouse
Conjugation HRP
Immunogen Antisera to Mouse IgG were raised by repeated immunisations of Goats with highly purified antigen.

Mouse IgG2c goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Mouse
Conjugation FITC

Mouse IgA rabbit polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse, Rat
Immunogen Mouse IgA paraproteins

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Human IgE goat polyclonal antibody, Biotin

Applications ELISA: 1/10,000-1/100,000. 
Western Blot: 1/10,000-1/100,000.
Reactivities Human, Monkey
Conjugation Biotin
Immunogen Native human IgE

Human IgG1 sheep polyclonal antibody, Purified

Applications ELISA: 1/4000-1/8000
Western Blot: 1/4000-1/8000
Reactivities Human
Immunogen Purified Human IgG1.

Goat IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Goat
Immunogen Purified goat IgM

Rabbit IgA goat polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Rabbit
Immunogen Purified rabbit IgA

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system.
Positive Control: Lymph node.
Reactivities Human
Immunogen Purified IgM.

Human IgA rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human

Bovine IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG in bovine serum or other body fluids or to determine its concentration.
To prepare an Immunoadsorbent for the purification of Bovine IgG from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Precipitin titre: 1/64 when tested against pooled normal bovine serum in agar-block Immunodiffusion titration.
Reactivities Bovine
Immunogen Highly purified normal IgG isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgA (Fc specific) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of IgA in Cat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Cat IgA from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/32 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Immunogen Highly purified normal IgA isolated from pooled cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal Cat serum.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Immunogen Purified normal IgG and fractions containing IgA and IgM and isolated from Cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgA (secretory) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of IgA in Cat milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Cat IgA from serum or exocrine secretions.
Antisera to IgA or sIgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/32 when tested against normal Cat serum in agar block titration.
Reactivities Feline
Immunogen Highly purified secretory IgA isolated from pooled Cat milk.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgG / Chicken IgY (F(ab)2 specific) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins as secondary antibody to precipitate the immunoglobulin in normal Chicken serum.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Chicken serum in agar block titration.
Reactivities Chicken
Immunogen Purified Fab of normal IgG isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgA (Fc specific) goat polyclonal antibody, Serum

Applications Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of IgA in Chicken serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Chicken IgA from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Chicken serum in agar block titration.
Reactivities Chicken
Immunogen Highly purified normal IgA isolated from pooled cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Immunogen Purified IgM isolated from Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgM (Fc specific) goat polyclonal antibody, Texas Red

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in chicken serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of chicken IgM from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Texas Red
Immunogen Highly purified normal IgM isolated from pooled chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Duck IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal duck serum.
Directions for use:
In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal duck serum in agar block titration.
Reactivities Duck
Immunogen Purified normal Ig fractions isolated from pooled duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Equine IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in horse serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgG from serum or plasma.
Directions for use:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1:32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
Reactivities Equine
Immunogen Highly purified normal IgG isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Equine IgG1 goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG1 in Horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgG1 from serum or plasma.
Directions for Use:
Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/32 when tested against pooled normal horse serum in agar block immunodiffusion titration.
Reactivities Equine
Immunogen Pools of purified normal IgG1 isolated from pooled serum from tetanus immunized Horses.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Equine IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.
Directions for use:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal horse serum in agar-block immunodiffusion titration.
Reactivities Equine
Immunogen Highly purified normal IgM isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgA (Secretory component) goat polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal human milk in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgD isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
In double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1:32 when tested against pooled an appropriate reference in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Highly purified monoclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgE in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Antibody titre: Precipitin titre not less than 1/16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Immunogen Highly purified monoclonal IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in human serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre Precipitin titre 1/32 when tested against pooled an appropriate reference in agar-block immunodiffusion titration.
Reactivities Human
Immunogen Highly purified monoclonal IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgE (Fc specific) goat polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgE in Rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Rat IgE from serum or plasma.
This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin Titre: 1/32 when tested against appropriate concentrations of rat IgE in agar-block immunodiffusion titration.
Reactivities Rat
Immunogen Highly purified homogenous IgE isolated from Rat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Rat
Immunogen Pools of purified homogenous IgE isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Mouse
Immunogen Pools of purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in rabbit serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgM from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Highly purified normal IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in rabbit serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rabbit IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended dilutions:
Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration.
Reactivities Rabbit
Immunogen Highly purified normal IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal rabbit serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion: a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal rabbit serum in agar block titration.
Reactivities Rabbit
Immunogen Purified Normal IgG and fractions containing IgA, and IgM isolated from Rabbit serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rat IgG2b (subclass specific) goat polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2b from serum or plasma.
Recommended working dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/32 when tested against pooled normal rat serum in agar block immunodiffusion titration.
Reactivities Rat
Immunogen Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG3 (subclass specific) goat polyclonal antibody, Serum

Applications Can be used in Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
In double radial immunodiffusion (Ouchterlony): use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not les than 1/32 when tested against pooled normal mouse serum in agar block immunodiffusion titration.
Reactivities Mouse
Immunogen Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG2 (subclass specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Pools of purified normal IgG2 isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG2 (subclass specific) sheep polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal goat serum in agar block titration.
Reactivities Bovine
Immunogen Pools of purified normal IgG2 isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgM from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/16 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Highly purified normal IgM isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Cat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma.
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially) diluted in 2 mm diameter peripheral wells.
Antibody titre: 1/32 (Precipitin titre) when tested against pooled normal Cat serum in agar-block immunodiffusion titration.
Reactivities Feline
Immunogen Highly purified normal IgA isolated from pooled Cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified normal IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgG from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in monkey serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgM from serum or plasma.
Directions for use:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgM isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in monkey serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fab specific) rabbit polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
Recommended working dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified Fab of normal Rhesus monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgA isolated from Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgG from serum or plasma.
Recommended working dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.