NS2a (NC_005147) Virus Tagged ORF Clone

CAT#: VC100599

Myc-DDK-tagged ORF clone of viral ORF for NS2a protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937948

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100599 represents NCBI reference of NP_937948 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTAGCCTATGCAGACAAGCCCAACCACTTCATTAACTTCCCTCTCACCCATTTCCAAGGGTTCG
  TGCTGAATTACAAGGGCCTCCAGTTCCAAATCCTGGACGAAGGCGTGGACTGTAAGATTCAAACAGCACC
  TCATATCTCTCTGACAATGCTCGATATTCAGCCCGAAGACTACAAGAGCGTTGATGTGGCCATTCAGGAG
  GTAATTGACGATATGCATTGGGGCGATGGATTCCAGATTAAGTTCGAGAATCCCCATATCCTGGGTCGCT
  GTATCGTGTTGGACGTGAAGGGGGTGGAGGAACTGCATGATGACCTCGTTAACTATATAAGAGATAAGGG
  CTGCGTCGCTGACCAAAGTAGAAAATGGATCGGCCACTGTACTATTGCCCAGCTCACCGATGCAGCACTT
  TCCATTAAAGAGAATGTAGATTTCATCAATTCAATGCAGTTTAATTATAAAATTACGATCAACCCCTCAA
  GCCCCGCACGGTTGGAAATAGTGAAGCTGGGAGCCGAGAAAAAAGATGGATTCTACGAGACAATAGTGTC
  CCATTGGATGGGCATCCGCTTTGAGTATACTTCCCCAACCGACAAGTTGGCTATGATCATGGGATACTGC
  TGCCTGGACGTCGTGAGAAAGGAGCTCGAAGAGGGGGATCTGCCCGAAAACGACGACGATGCCTGGTTCA
  AGCTGTCTTACCACTACGAAAATAACAGCTGGTTTTTTAGGCATGTGTATAGGAAATCCTTCCACTTTAG
  AAAGGCTTGCCAGAACCTGGACTGCAATTGTCTGGGCTTCTACGAGTCATCTGTTGAAGAATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100599 representing NP_937948
  Red=Cloning sites Green=Tags
  
  MAVAYADKPNHFINFPLTHFQGFVLNYKGLQFQILDEGVDCKIQTAPHISLTMLDIQPEDYKSVDVAIQE
  VIDDMHWGDGFQIKFENPHILGRCIVLDVKGVEELHDDLVNYIRDKGCVADQSRKWIGHCTIAQLTDAAL
  SIKENVDFINSMQFNYKITINPSSPARLEIVKLGAEKKDGFYETIVSHWMGIRFEYTSPTDKLAMIMGYC
  CLDVVRKELEEGDLPENDDDAWFKLSYHYENNSWFFRHVYRKSFHFRKACQNLDCNCLGFYESSVEEY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937948
• RefSeq ORF: 834
• Locus ID: 2648207
• MW: 32.3 kDa