NS2 (NC_005147) Virus Tagged ORF Clone

CAT#: VC100602

Myc-DDK-tagged ORF clone of viral ORF for NS2 protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937951

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100602 represents NCBI reference of NP_937951 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATATGGAGACCCGAGAAGAAGTATCTCAGGTACATCAATGGGTTTAATGTCAGCGAACTGGAGG
  ACGCTTGCTTCAAATTCAACTATCAGTTCCCCAAGGTGGGATACTGCCGCGTGCCAAGCCATGCATGGTG
  TCGAAACCAAGGGAGATTCTGTGCAACTTTCACACTGTACGGCAAAAGTAAGCATTACGACAAATATTTC
  GGGGTGATAAATGGATTTACAGCCTTTGCGAACACCGTAGAGGATGCAGTGAATAAGCTGGTCTTTCTGG
  CAGTCGATTTCATCACCTGGCGGAGGCAGGAGCTCAACGTTTATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100602 representing NP_937951
  Red=Cloning sites Green=Tags
  
  MDIWRPEKKYLRYINGFNVSELEDACFKFNYQFPKVGYCRVPSHAWCRNQGRFCATFTLYGKSKHYDKYF
  GVINGFTAFANTVEDAVNKLVFLAVDFITWRRQELNVYG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 327 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937951
• RefSeq ORF: 327
• Locus ID: 2648211
• MW: 12.9 kDa