NS3 (NC_005147) Virus Tagged ORF Clone

CAT#: VC100603

Myc-DDK-tagged ORF clone of viral ORF for NS3 protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937952

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100603 represents NCBI reference of NP_937952 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCATGGCCGATGCCTACCTCGCCGACACCGTGTGGTACGTCGGACAGATTATTTTCATCGTGGCAA
  TCTGCCTGCTCGTGACCATCGTGGTTGTCGCCTTCCTGGCTACCTTCAAGCTTTGCATTCAGCTGTGTGG
  GATGTGTAATACCCTGGTGCTGTCACCCAGCATCTATGTGTTCAATAGAGGGCGCCAGTTTTACGAATTC
  TACAACGACGTCAAGCCACCTGTGTTGGACGTGGACGACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100603 representing NP_937952
  Red=Cloning sites Green=Tags
  
  MFMADAYLADTVWYVGQIIFIVAICLLVTIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNRGRQFYEF
  YNDVKPPVLDVDDV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 252 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937952
• RefSeq ORF: 252
• Locus ID: 2648206
• MW: 9.5 kDa