NS3 (NC_005147) Virus Tagged ORF Clone

CAT#: VC100603

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for NS3 protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937952


  View other clones from "HCoV-OC43" (7)

Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol NS3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100603 represents NCBI reference of NP_937952 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCATGGCCGATGCCTACCTCGCCGACACCGTGTGGTACGTCGGACAGATTATTTTCATCGTGGCAA
TCTGCCTGCTCGTGACCATCGTGGTTGTCGCCTTCCTGGCTACCTTCAAGCTTTGCATTCAGCTGTGTGG
GATGTGTAATACCCTGGTGCTGTCACCCAGCATCTATGTGTTCAATAGAGGGCGCCAGTTTTACGAATTC
TACAACGACGTCAAGCCACCTGTGTTGGACGTGGACGACGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100603 representing NP_937952
Red=Cloning sites Green=Tags

MFMADAYLADTVWYVGQIIFIVAICLLVTIVVVAFLATFKLCIQLCGMCNTLVLSPSIYVFNRGRQFYEF
YNDVKPPVLDVDDV

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_005147
ORF Size 252 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_005147.1, NP_937952
RefSeq ORF 252
Locus ID 2648206
MW 9.5 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.