HE (NC_005147) Virus Tagged ORF Clone

CAT#: VC100600

Myc-DDK-tagged ORF clone of viral ORF for HE protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937949

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100600 represents NCBI reference of NP_937949 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTGTTGCCGCGCTTTATCCTGGTCAGTTGCATAATCGGGTCATTGGGCTTCTATAATCCCCCTA
  CAAACGTGGTGAGCCATGTCAACGGCGATTGGTTCCTTTTTGGCGACAGCCGAAGCGATTGTAATCATAT
  AGTGAACATCAACCCCCACAACTACAGCTATATGGATCTGAACCCCGTACTTTGCGATTCTGGGAAGATT
  TCAAGCAAGGCCGGGAATAGTATCTTCCGAAGCTTCCACTTCACAGATTTCTACAACTACACCGGAGAAG
  GCCAGCAGATCATATTCTATGAAGGGGTGAACTTCACTCCCTACCATGCCTTCAAATGCAACCGAAGCGG
  CTCAAACGACATCTGGATGCAGAACAAAGGCCTGTTCTATACCCAGGTGTACAAGAATATGGCGGTCTAC
  AGATCTCTCACCTTTGTCAATGTTCCATATGTGTATAATGGGAGCGCCCAGGCCACTGCCCTCTGCAAAT
  CTGGCAGCCTGGTGCTCAATAACCCCGCATACATTGCCCCGCAGGCCAATTCAGGCGACTACTATTATAA
  GGTGGAGGCCGACTTCTATCTGTCAGGCTGCGATGAATACATCGTTCCCTTGTGCATTTTTAACGGTAAG
  TTTCTGTCCAACACAAAGTACTACGACGACTCACAGTATTATTTCAACAAGGATACTGGCGTGATATACG
  GACTCAACTCCACTGAAACCATCACGACCGGCTTCGATTTGAATTGCTACTATCTTGTGCTTCCCAGTGG
  GAATTACCTGGCCATTTCAAACGAACTGTTGCTGACCGTTCCTACCAAGGCCATCTGCCTCAACAAAAGA
  AAGGATTTTACCCCCGTGCAGGTGGTGGACAGTCGCTGGAACAACGCCAGGCAGTCAGACAACATGACTG
  CAGTGGCTTGTCAACCTCCTTACTGCTACTTCCGCAACTCTACTACTAATTATGTAGGTGTGTATGATAT
  TAATCATGGGGATGCTGGGTTTACATCCATCCTGAGCGGGCTCCTGTACAATAGCCCATGCTTTTCTCAG
  CAGGGCGTATTTCGGTACGACAATGTTAGTTCCGTGTGGCCGCTTTACCCATATGGTCGCTGTCCCACTG
  CCGCCGATATCAATATACCAGATCTCCCGATATGCGTGTATGATCCCTTGCCGGTTATCCTCCTCGGTAT
  TCTTCTGGGAGTGGCAATTGTGATCATAGTGGTGCTTCTTCTGTACTTCATGGTCGATAACGTAACTCGC
  TTGCACGACGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100600 representing NP_937949
  Red=Cloning sites Green=Tags
  
  MFLLPRFILVSCIIGSLGFYNPPTNVVSHVNGDWFLFGDSRSDCNHIVNINPHNYSYMDLNPVLCDSGKI
  SSKAGNSIFRSFHFTDFYNYTGEGQQIIFYEGVNFTPYHAFKCNRSGSNDIWMQNKGLFYTQVYKNMAVY
  RSLTFVNVPYVYNGSAQATALCKSGSLVLNNPAYIAPQANSGDYYYKVEADFYLSGCDEYIVPLCIFNGK
  FLSNTKYYDDSQYYFNKDTGVIYGLNSTETITTGFDLNCYYLVLPSGNYLAISNELLLTVPTKAICLNKR
  KDFTPVQVVDSRWNNARQSDNMTAVACQPPYCYFRNSTTNYVGVYDINHGDAGFTSILSGLLYNSPCFSQ
  QGVFRYDNVSSVWPLYPYGRCPTAADINIPDLPICVYDPLPVILLGILLGVAIVIIVVLLLYFMVDNVTR
  LHDA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937949
• RefSeq ORF: 1272
• Locus ID: 2648209
• MW: 47.7 kDa