S (NC_005147) Virus Tagged ORF Clone

CAT#: VC100601

Myc-DDK-tagged ORF clone of viral ORF for S protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937950

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100601 represents NCBI reference of NP_937950 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTGATTCTGCTGATCAGTCTGCCCACCGCCTTTGCTGTGATTGGAGACCTTAAATGTACTTCAG
  ATACGTCATATATCAATGACAAAGACACCGGCCCCCCACCAATTTCAACCGACACAGTTGATGTTACAAA
  CGGACTGGGCACATACTATGTGCTCGACCGCGTCTATCTGAACACAACCCTGTTTCTGAATGGTTATTAT
  CCTACGTCCGGCTCCACCTACCGCAACATGGCGCTTAAGGGATCCGTGTTGCTGTCCCGGCTTTGGTTCA
  AACCACCATTCTTGAGTGATTTTATTAATGGGATCTTTGCTAAGGTCAAAAACACCAAAGTTATTAAAGA
  TCGGGTTATGTACTCCGAGTTCCCTGCTATTACCATCGGAAGTACATTCGTGAACACATCTTATTCCGTA
  GTCGTCCAGCCTCGGACCATCAACAGCACTCAGGACGGATACAATAAGCTGCAGGGACTCCTGGAGGTGA
  GCGTCTGCCAGTACAACATGTGCGAGTACCCTCAGACCATTTGTCATCCAAACCTGGGAAACCACCGCAA
  AGAACTGTGGCATTTGGACACTGGAGTTGTTAGCTGTCTGTACAAAAGAAATTTCACCTATGACGTCAAT
  GCCGACTATCTGTACTTCCATTTTTATCAGGAGGGGGGTACGTTCTACGCCTACTTTACCGACACCGGAG
  TCGTCACCAAATTCCTTTTTAATGTGTACCTCGGAATGGCCCTGAGCCATTACTATGTTATGCCACTGAC
  CTGCAACAGCAAAGTTAAGAATGGGTTTACCCTCGAATACTGGGTAACCCCCCTCACAAGCCGGCAATAT
  CTCCTTGCCTTTAACCAAGACGGCATTATCTTCAACGCAGTAGACTGCATGTCTGACTTCATGTCTGAAA
  TCAAGTGTAAAACGCAATCAATTGCACCCCCCACAGGTGTGTACGAACTCAACGGTTATACTGTGCAGCC
  CATCGCTGATGTGTACAGGAGGAAATTGAACCTGCCCAACTGTAATATTGAGGCCTGGCTTAACGATAAG
  TCAGTCCCATCTCCTCTGAATTGGGAGAGAAAGACATTCAGCAACTGCAATTTCAACATGTCCTCACTTA
  TGTCATTCATTCAGGCTGACAGTTTCACGTGCAACAACATTGACGCTGCTAAGATTTATGGAATGTGTTT
  CAGTAGCATCACTATCGACAAATTCGCAATCCCAAATGGTAGGAAGGTGGACCTCCAGTTGGGCAACCTG
  GGCTATCTGCAGTCATTCAACTATAGAATCGACACCACAGCCACTTCCTGCCAGCTGTACTACAACTTGC
  CCGCTGCCAATGTCTCTGTCAGCAGGTTTAATCCTTCTACGTGGAATAAAAGATTTGGATTTATTGAGGA
  TTCTGTTTTTAAACCACGGCCGGCGGGCGTTCTGACTAACCATGACGTGGTATACGCACAACACTGCTTT
  AAGGCACCCAAAAACTTTTGCCCCTGCAAACTCAATGGAAGCTGCGTAGGCTCTGGTCCAGGAAAAAATA
  ATGGTATAGGTACATGCCCCGCGGGCACAAACTACCTGACTTGTGACAATCTGTGTACACCTGACCCGAT
  TACTTTCAAGGCTACAGGCACTTATAAGTGCCCCCAGACTAAATCTCTGGTCGGCATTGGAGAGCACTGT
  AGCGGCCTGGCAGTGAAAAGCGATTATTGCGGAGGTAATAGCTGTACCTGCAGACCACAGGCTTTCCTTG
  GATGGAGCGCTGACAGTTGCCTCCAAGGCGACAAGTGCAACATTTTCGCCAATTTCATTCTCCACGACGT
  GAATAGCGGCCTCACCTGTAGCACTGACCTGCAGAAAGCTAACACTGACATTATTCTCGGGGTCTGCGTG
  AATTACGATCTTTACGGGATTCTGGGCCAGGGGATTTTCGTCGAGGTGAACGCTACCTATTATAACAGCT
  GGCAGAATCTCCTTTACGATAGCAACGGCAACCTCTACGGCTTTCGCGACTATATTACAAATCGAACCTT
  CATGATACGGTCCTGTTACTCCGGCCGCGTGAGCGCGGCCTTTCACGCCAATTCTAGTGAACCTGCACTT
  CTGTTTAGGAACATCAAGTGCAATTACGTGTTCAATAACAGTCTCACTCGGCAGCTGCAGCCCATCAACT
  ACTTTGATAGCTACCTTGGTTGCGTCGTGAACGCCTACAATTCCACCGCAATAAGCGTGCAGACATGTGA
  TTTGACCGTGGGCTCCGGATATTGCGTTGACTACTCAAAGAACCGACGCAGCCGGGGAGCCATCACCACG
  GGTTACCGCTTTACCAACTTTGAACCATTCACAGTCAATAGCGTGAACGACAGCCTCGAACCAGTGGGTG
  GTCTGTACGAGATTCAAATACCTAGCGAGTTCACCATAGGCAACATGGAAGAATTTATTCAGACGAGTAG
  TCCTAAAGTTACAATAGACTGTGCAGCTTTCGTCTGCGGCGATTATGCGGCCTGTAAAAGTCAACTGGTG
  GAGTACGGCTCCTTCTGTGATAACATCAATGCCATACTGACTGAGGTGAACGAGCTGCTTGACACTACAC
  AACTGCAGGTGGCAAATAGCCTGATGAATGGCGTGACGCTCTCAACCAAGCTGAAGGACGGGGTCAACTT
  TAATGTGGATGACATTAACTTTAGCCCTGTGCTCGGTTGTCTGGGGTCCGAATGTAGTAAGGCGAGCTCC
  CGGTCTGCAATAGAGGATCTGCTGTTTGACAAAGTCAAACTTAGCGATGTGGGGTTCGTTGAAGCATATA
  ACAATTGCACAGGTGGGGCTGAAATTCGCGATCTGATTTGCGTGCAATCTTATAAAGGCATCAAGGTGCT
  GCCCCCTTTGCTGTCCGAAAATCAGATTTCCGGCTACACTCTGGCCGCCACCTCCGCCAGCCTCTTTCCT
  TTGTGGACTGCCGCTGCAGGGGTGCCCTTCTATCTGAACGTGCAGTACAGGATCAATGGATTGGGTGTAA
  CGATGGATGTGCTGTCACAGAACCAGAAACTTATTGCAAACGCTTTTAATAACGCCCTCTATGCCATACA
  GGAGGGATTCGACGCTACTAATTCCGCGCTCGTCAAAATTCAGGCAGTCGTGAATGCGAATGCGGAGGCC
  CTGAATAATCTCCTCCAGCAGCTTTCCAACAGATTCGGTGCAATATCTGCGAGTCTGCAAGAAATATTGT
  CTCGCCTGGACGCGCTCGAGGCAGAAGCGCAGATCGACAGACTGATCAACGGTAGATTGACCGCTCTGAA
  TGCGTATGTGTCACAACAGCTGTCTGACTCCACTCTTGTCAAGTTTTCCGCCGCGCAGGCCATGGAGAAG
  GTCAACGAATGCGTGAAATCACAAAGTAGCCGAATAAACTTTTGCGGAAACGGAAACCACATCATTTCTC
  TGGTCCAGAACGCTCCATACGGACTGTACTTTATTCACTTCAGCTATGTCCCCACAAAATACGTCACTGC
  CCGCGTCTCACCAGGGCTGTGCATCGCTGGCGATCGGGGCATTGCTCCTAAGTCAGGATACTTTGTGAAC
  GTTAACAATACTTGGATGTATACTGGTAGCGGGTATTACTACCCCGAACCTATAACTGAGAATAACGTGG
  TGGTGATGAGCACATGTGCCGTGAACTACACCAAGGCTCCTTACGTGATGCTTAATACGAGCATTCCAAA
  TCTCCCGGATTTTAAAGAGGAGCTGGATCAGTGGTTTAAGAACCAGACAAGTGTGGCCCCCGACCTGAGC
  CTCGACTATATAAATGTTACCTTTCTGGATTTGCAGGTGGAGATGAATAGACTGCAGGAGGCCATCAAGG
  TCCTGAATCAGAGCTACATCAACCTTAAGGATATTGGCACATACGAGTACTACGTGAAATGGCCATGGTA
  TGTCTGGCTGCTCATCTGCCTCGCTGGCGTGGCTATGTTGGTCCTTCTGTTCTTCATCTGCTGTTGTACC
  GGATGCGGAACATCCTGCTTCAAGAAGTGCGGAGGATGCTGCGATGATTATACGGGCTATCAGGAGCTGG
  TGATCAAAACTTCCCATGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100601 representing NP_937950
  Red=Cloning sites Green=Tags
  
  MFLILLISLPTAFAVIGDLKCTSDTSYINDKDTGPPPISTDTVDVTNGLGTYYVLDRVYLNTTLFLNGYY
  PTSGSTYRNMALKGSVLLSRLWFKPPFLSDFINGIFAKVKNTKVIKDRVMYSEFPAITIGSTFVNTSYSV
  VVQPRTINSTQDGYNKLQGLLEVSVCQYNMCEYPQTICHPNLGNHRKELWHLDTGVVSCLYKRNFTYDVN
  ADYLYFHFYQEGGTFYAYFTDTGVVTKFLFNVYLGMALSHYYVMPLTCNSKVKNGFTLEYWVTPLTSRQY
  LLAFNQDGIIFNAVDCMSDFMSEIKCKTQSIAPPTGVYELNGYTVQPIADVYRRKLNLPNCNIEAWLNDK
  SVPSPLNWERKTFSNCNFNMSSLMSFIQADSFTCNNIDAAKIYGMCFSSITIDKFAIPNGRKVDLQLGNL
  GYLQSFNYRIDTTATSCQLYYNLPAANVSVSRFNPSTWNKRFGFIEDSVFKPRPAGVLTNHDVVYAQHCF
  KAPKNFCPCKLNGSCVGSGPGKNNGIGTCPAGTNYLTCDNLCTPDPITFKATGTYKCPQTKSLVGIGEHC
  SGLAVKSDYCGGNSCTCRPQAFLGWSADSCLQGDKCNIFANFILHDVNSGLTCSTDLQKANTDIILGVCV
  NYDLYGILGQGIFVEVNATYYNSWQNLLYDSNGNLYGFRDYITNRTFMIRSCYSGRVSAAFHANSSEPAL
  LFRNIKCNYVFNNSLTRQLQPINYFDSYLGCVVNAYNSTAISVQTCDLTVGSGYCVDYSKNRRSRGAITT
  GYRFTNFEPFTVNSVNDSLEPVGGLYEIQIPSEFTIGNMEEFIQTSSPKVTIDCAAFVCGDYAACKSQLV
  EYGSFCDNINAILTEVNELLDTTQLQVANSLMNGVTLSTKLKDGVNFNVDDINFSPVLGCLGSECSKASS
  RSAIEDLLFDKVKLSDVGFVEAYNNCTGGAEIRDLICVQSYKGIKVLPPLLSENQISGYTLAATSASLFP
  LWTAAAGVPFYLNVQYRINGLGVTMDVLSQNQKLIANAFNNALYAIQEGFDATNSALVKIQAVVNANAEA
  LNNLLQQLSNRFGAISASLQEILSRLDALEAEAQIDRLINGRLTALNAYVSQQLSDSTLVKFSAAQAMEK
  VNECVKSQSSRINFCGNGNHIISLVQNAPYGLYFIHFSYVPTKYVTARVSPGLCIAGDRGIAPKSGYFVN
  VNNTWMYTGSGYYYPEPITENNVVVMSTCAVNYTKAPYVMLNTSIPNLPDFKEELDQWFKNQTSVAPDLS
  LDYINVTFLDLQVEMNRLQEAIKVLNQSYINLKDIGTYEYYVKWPWYVWLLICLAGVAMLVLLFFICCCT
  GCGTSCFKKCGGCCDDYTGYQELVIKTSHDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 4083 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937950
• RefSeq ORF: 4083
• Locus ID: 2648208
• MW: 151.0 kDa