M (NC_005147) Virus Tagged ORF Clone

CAT#: VC100604

Myc-DDK-tagged ORF clone of viral ORF for M protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937953

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100604 represents NCBI reference of NP_937953 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGCAAAACCACACCCGCCCCTGTTTATATCTGGACTGCAGATGAGGCCATCAAGTTTCTGAAGG
  AATGGAACTTTTCCCTGGGAATAATTCTCCTGTTCATTACAATCATACTTCAGTTTGGGTATACTTCCAG
  ATCTATGTTTGTTTACGTCATAAAAATGATTATTCTGTGGCTGATGTGGCCACTCACAATTATCCTGACC
  ATTTTCAACTGTGTTTACGCACTCAACAATGTATATCTGGGACTGAGTATTGTGTTTACAATTGTGGCCA
  TAATTATGTGGATTGTGTACTTCGTCAATTCAATTCGACTGTTCATTCGCACCGGGTCCTTCTGGTCCTT
  TAATCCAGAGACAAATAATTTGATGTGCATTGATATGAAGGGAACAATGTACGTGCGGCCAATCATCGAG
  GACTACCACACCCTCACTGTTACCATTATTAGGGGGCATCTGTATATTCAGGGAATCAAGCTCGGAACCG
  GCTACAGCCTTGCTGACCTGCCAGCCTACATGACCGTCGCTAAGGTGACCCATCTGTGTACCTATAAAAG
  AGGGTTTCTGGATAGGATATCTGATACAAGTGGTTTCGCAGTGTACGTAAAAAGCAAGGTGGGCAACTAT
  AGGCTCCCTTCCACCCAGAAAGGTTCTGGCATGGACACCGCACTCCTGAGGAACAACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100604 representing NP_937953
  Red=Cloning sites Green=Tags
  
  MSSKTTPAPVYIWTADEAIKFLKEWNFSLGIILLFITIILQFGYTSRSMFVYVIKMIILWLMWPLTIILT
  IFNCVYALNNVYLGLSIVFTIVAIIMWIVYFVNSIRLFIRTGSFWSFNPETNNLMCIDMKGTMYVRPIIE
  DYHTLTVTIIRGHLYIQGIKLGTGYSLADLPAYMTVAKVTHLCTYKRGFLDRISDTSGFAVYVKSKVGNY
  RLPSTQKGSGMDTALLRNNI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 690 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937953
• RefSeq ORF: 690
• Locus ID: 2648205
• MW: 26.3 kDa