N (NC_005147) Virus Tagged ORF Clone

CAT#: VC100605

Myc-DDK-tagged ORF clone of viral ORF for N protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937954

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100605 represents NCBI reference of NP_937954 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTTACCCCCGGGAAACAGTCTAGTTCACGGGCTTCTAGCGGCAATAGGTCAGGCAATGGAATCC
  TCAAATGGGCTGACCAGTCAGACCAATTCCGGAACGTCCAGACTCGAGGACGCAGAGCACAACCAAAACA
  AACCGCTACTTCACAGCAGCCCAGTGGGGGGAACGTGGTTCCCTATTATTCCTGGTTTTCAGGAATCACC
  CAGTTTCAAAAGGGTAAGGAGTTCGAATTCGTCGAGGGACAAGGAGTTCCCATCGCTCCCGGCGTTCCCG
  CTACTGAGGCGAAGGGTTATTGGTACAGGCACAACAGGAGGAGCTTCAAGACTGCCGATGGAAATCAGAG
  ACAGCTTCTCCCTCGGTGGTACTTCTACTATTTGGGAACAGGGCCTCACGCTAAGGACCAATACGGAACA
  GACATTGATGGCGTATATTGGGTCGCCAGCAACCAGGCCGACGTGAACACCCCAGCTGATATCGTTGACC
  GGGACCCATCCAGCGATGAAGCAATCCCAACACGGTTTCCACCGGGAACAGTGCTGCCGCAGGGCTACTA
  TATTGAAGGAAGCGGGAGATCAGCGCCAAATTCCAGATCCACGTCCCGAACTAGCTCCAGGGCCTCAAGC
  GCCGGATCCCGCTCAAGAGCCAACAGCGGGAACCGAACCCCAACGAGTGGCGTAACACCAGATATGGCTG
  ACCAAATCGCTAGTCTTGTGCTGGCAAAGCTCGGTAAGGACGCTACAAAACCACAGCAAGTCACTAAACA
  TACAGCCAAAGAAGTTCGCCAAAAGATCCTCAATAAACCCAGGCAGAAGCGATCACCAAACAAACAGTGT
  ACTGTGCAACAATGCTTCGGGAAACGCGGTCCGAATCAAAATTTCGGCGGCGGTGAGATGCTGAAACTGG
  GCACATCAGACCCACAGTTCCCAATATTGGCTGAGCTGGCACCTACTGCCGGGGCATTCTTCTTTGGCAG
  CCGATTGGAGCTTGCCAAAGTGCAGAACTTGAGTGGCAATCCTGATGAGCCCCAGAAAGATGTGTACGAG
  CTTCGCTACAATGGAGCCATTAGATTCGACAGTACATTGTCAGGCTTTGAGACCATCATGAAAGTTTTGA
  ACGAAAATCTGAACGCTTACCAACAGCAGGATGGCATGATGAATATGTCTCCGAAGCCACAGCGGCAAAG
  AGGACATAAAAATGGACAGGGAGAGAACGACAACATAAGTGTCGCAGTGCCGAAGAGCAGGGTCCAGCAG
  AACAAAAGCCGAGAACTTACAGCAGAAGACATCTCCCTGCTGAAGAAGATGGATGAACCATATACGGAGG
  ATACAAGCGAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100605 representing NP_937954
  Red=Cloning sites Green=Tags
  
  MSFTPGKQSSSRASSGNRSGNGILKWADQSDQFRNVQTRGRRAQPKQTATSQQPSGGNVVPYYSWFSGIT
  QFQKGKEFEFVEGQGVPIAPGVPATEAKGYWYRHNRRSFKTADGNQRQLLPRWYFYYLGTGPHAKDQYGT
  DIDGVYWVASNQADVNTPADIVDRDPSSDEAIPTRFPPGTVLPQGYYIEGSGRSAPNSRSTSRTSSRASS
  AGSRSRANSGNRTPTSGVTPDMADQIASLVLAKLGKDATKPQQVTKHTAKEVRQKILNKPRQKRSPNKQC
  TVQQCFGKRGPNQNFGGGEMLKLGTSDPQFPILAELAPTAGAFFFGSRLELAKVQNLSGNPDEPQKDVYE
  LRYNGAIRFDSTLSGFETIMKVLNENLNAYQQQDGMMNMSPKPQRQRGHKNGQGENDNISVAVPKSRVQQ
  NKSRELTAEDISLLKKMDEPYTEDTSEI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937954
• RefSeq ORF: 1344
• Locus ID: 2648210
• MW: 49.5 kDa