N2 (NC_005147) Virus Tagged ORF Clone

CAT#: VC100606

Myc-DDK-tagged ORF clone of viral ORF for N2 protein [Human coronavirus OC43], codon optimized for human cell expression, NP_937955

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100606 represents NCBI reference of NP_937955 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACATCAGTGAACTGCTGCCACGATGGCATCTTCACGATCTGGGAGCAAGACCGCATGCTCAAAA
  CCAGCACAGCACCAATTCTGACCGAAAGTACTGGGTCTCTTGCGACTCGGCTCATGTCCATCCCCCGGCT
  TACACTCTCTATTGGGACCCAGGTTGCCATGCGATTGTTTCGGCTCGGGTTCCGGCTGGCTCGGTATTCC
  CTGAGGGTAACCATTCTCAAGGCGCAGGAAGGACTGCTGCTGATCCCGGACCTTCTTCGAGCGCACCCAG
  CGGAACCCCTGGTACAGGACCGCGTTGTGGAGCCTATCCTGGCCATCGAACCACTGCCCCTCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100606 representing NP_937955
  Red=Cloning sites Green=Tags
  
  MATSVNCCHDGIFTIWEQDRMLKTSTAPILTESTGSLATRLMSIPRLTLSIGTQVAMRLFRLGFRLARYS
  LRVTILKAQEGLLLIPDLLRAHPAEPLVQDRVVEPILAIEPLPLV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_005147
• ORF Size: 345 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_005147.1, NP_937955
• RefSeq ORF: 345
• Locus ID: 2648212
• MW: 12.8 kDa