VP4 (NC_014336) Virus Tagged ORF Clone

CAT#: VC100692

Myc-DDK-tagged ORF clone of viral ORF for VP4 [Human enterovirus 109], codon optimized for human cell expression, YP_003795175

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100692 represents NCBI reference of YP_003795175 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGGCGCTCAAGTGTCTGCTCAGAACAACGGGACTCACGAGAACTCCAATTCAGCCAGTGGAGGGA
  GCACAATAAACTATACCACCATCAACTACTATAAAGATAGCGCATCCAACGCAGCAAGCAAGCTCGATTT
  TTCTCAAGATCCATCCAAGTTCACTGAACCTGTGAAGGATGTCATGATTAAAACCGCTCCGGCACTGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100692 representing YP_003795175
  Red=Cloning sites Green=Tags
  
  MMGAQVSAQNNGTHENSNSASGGSTINYTTINYYKDSASNAASKLDFSQDPSKFTEPVKDVMIKTAPALN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 210 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795175
• RefSeq ORF: 210
• MW: 7.4 kDa