3C (NC_014336) Virus Tagged ORF Clone

CAT#: VC100701

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus 109], codon optimized for human cell expression, YP_003795184

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100701 represents NCBI reference of YP_003795184 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCGCCTTCGACTACGCTGTGGCCATGGCAAGACGGAATATCCTTACCGCCACAACAGACAAGG
  GTGAGTTCACCATGCTGGGGATCTATGACCGAGTGGCAGTTCTCCCAACTCACAGCTCTCCAGGCGACAC
  TGTGGTTATTGCCGGAAAGGAGGTGAAGGTCAAAGATGCAAGACGCCTTGTGGACACGAACGATGTGAAT
  CTGGAGGTGACGATTGTTGAACTGGACCGGAACGAGAAATTCAGGGACATAAGGGCGCATATCCCGCCCA
  CTATTTGTGAGACGAGTGAAGCAATACTCGCAGTCAACACCACAAGATTTCCAAACATGTACATACCGGT
  GGGGGCCGTAGCAGAGCAAGGCATGTTGAATCTCGGCGGCAGACCTACTAACCGAACACTGATGTACAAC
  TTCCCAACGAAGGCCGGCCAATGCGGGGGAGTAGTAATGTCTACAGGTAAGGTGATCGGGATTCACGTCG
  GAGGCAACGGATCTCACGGATTTGCCGCTGCACTCAAGCGGAGTTATTTCACATCAGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100701 representing YP_003795184
  Red=Cloning sites Green=Tags
  
  MGPAFDYAVAMARRNILTATTDKGEFTMLGIYDRVAVLPTHSSPGDTVVIAGKEVKVKDARRLVDTNDVN
  LEVTIVELDRNEKFRDIRAHIPPTICETSEAILAVNTTRFPNMYIPVGAVAEQGMLNLGGRPTNRTLMYN
  FPTKAGQCGGVVMSTGKVIGIHVGGNGSHGFAAALKRSYFTSEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 552 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795184
• RefSeq ORF: 552
• MW: 19.9 kDa