2B (NC_014336) Virus Tagged ORF Clone

CAT#: VC100697

Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus 109], codon optimized for human cell expression, YP_003795180

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100697 represents NCBI reference of YP_003795180 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCACAGATTACATCTCTGGCCTGGGGGCTGCATTTGGCGCGGGGTTTTCCACCGAAATCAGTT
  CCGGCATACAGGAACTGCAGGAGACTGTGCAGAATGTCATCACAGAAAAACTTCTCAAGAATCTGGTTAA
  GATCGTATCCGCCTTGGTCATTGTTGCCAGAAACTACGAAGACAGCATTACCGTGCTCGCGACTCTGTCA
  CTGTTGGGATGCGACAGTAGCCCATGGCATTGGCTGAAAGAGAAAGTGTGTAATATTCTCGGTATACCTT
  ACGCTATGAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100697 representing YP_003795180
  Red=Cloning sites Green=Tags
  
  MGITDYISGLGAAFGAGFSTEISSGIQELQETVQNVITEKLLKNLVKIVSALVIVARNYEDSITVLATLS
  LLGCDSSPWHWLKEKVCNILGIPYAMKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 294 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795180
• RefSeq ORF: 294
• MW: 10.6 kDa