VP2 (NC_014336) Virus Tagged ORF Clone

CAT#: VC100693

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human enterovirus 109], codon optimized for human cell expression, YP_003795176

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100693 represents NCBI reference of YP_003795176 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCAAACATCGAGGCTTGCGGGTATTCTGATCGCGTGTTGCAGCTGACCCTCGGGAATTCAACAA
  TAACGACCCAGGAGGCGGCCAATGCCGTTATAGGCTACGGCGAGTGGCCCCAGTTCCTGAACTCCGCCGA
  TGCAAATCCAGTGGACGCACCCACTCTGCCGGACAGCTCATCCAATAGGTTCTACACCCTGGAGTCAGTG
  GAGTGGCAATCCAGTTCTCGCGGGTGGTGGTGGAAACTGCCCGATGCGCTTAAAGACATGGGTATGTTTG
  GTCAGAATATGTACTACCACTATCTCGGTAGAGCTGGCTACACGGTTCATGTGCAATGCAATGCAAGTAA
  ATTTCACCAAGGCGCCCTCTTCGTTGCCGCCATACCCGAGTTCATGATGGCTTCTAATTCCGCAGATCAA
  ACGGGCGGCATTACCTATGAGAAAGCCAATCCTGGCGAGGCAGGCGGCAAGTTCCAGGCATCATTTACCC
  CTAGTGCAGAGGACGGTAAGAAAAACACTTTTGCCCCGGTTGATTGGCTCTTGGGATGCGGGGTGATGGC
  AGGAAATATTACCGTTTTTCCCCACCAGATCATCAATCTGCGCACAAACAATTGCGCCACATTGGTTCTG
  CCCTACGTAAATAGCTCTGCCACCGACTCCATGGCCAAACATAACAATTGGGGAATTGTGATCCTTCCGT
  TTGTACCCCTGCAGTACGAAGACGGAGCTTCCACTGCCATTCCCATCACAGTAACCGTAGCGCCCATGTG
  TTGTGAATTCAATGGACTGAGAGCACAAACGAACCCTGTGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100693 representing YP_003795176
  Red=Cloning sites Green=Tags
  
  MSPNIEACGYSDRVLQLTLGNSTITTQEAANAVIGYGEWPQFLNSADANPVDAPTLPDSSSNRFYTLESV
  EWQSSSRGWWWKLPDALKDMGMFGQNMYYHYLGRAGYTVHVQCNASKFHQGALFVAAIPEFMMASNSADQ
  TGGITYEKANPGEAGGKFQASFTPSAEDGKKNTFAPVDWLLGCGVMAGNITVFPHQIINLRTNNCATLVL
  PYVNSSATDSMAKHNNWGIVILPFVPLQYEDGASTAIPITVTVAPMCCEFNGLRAQTNPVLQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795176
• RefSeq ORF: 816
• MW: 29.6 kDa