2C (NC_014336) Virus Tagged ORF Clone

CAT#: VC100698

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 109], codon optimized for human cell expression, YP_003795181

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100698 represents NCBI reference of YP_003795181 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTCTTGGATGAAGAAGTTCAGTGACGCTTGTAACGCCGCTAAAGGCCTGGAGTGGATCTCCC
  AAAAGATAGGTAAATTTATTGATTGGATCAAGACAAAAATCCTTCCTGAGGCTCAGGACAAGGTGGAATA
  CCTGAGCAAACTGAAGCACCTGGACACTCTGGAGAATCAAGTGACCACTCTCCATCAGAGTTGCCCCTCC
  CAGGAGGATCAGGAGACGCTCTTCAATAACATTAGGTGGCTTGCCATAAAGGCCAGAAAGTTTGCCCCTC
  TGTACGCTAGCGAGGCACGCCGAATCTTTAAGATGGAATCAACTATCAACAATTACATTCAATTTAAGAC
  CAAGCACCGCATCGAACCTGTTTGTCTTTTGATCCACGGCACCCCGGGAACAGGAAAGTCTATCGCCACA
  GGACTGATTGGTAGAGCAATAGCAAAGCAGGCTAACACGAGCACATATTCCCTCCCTCCTGATCCGAGTC
  ACTTTGATGGATACAAACAGCAGGGCGTGGTGATTATGGATGACCTCAATCAGAACCCTGATGGGGAAGA
  CATGAAGCTGTTTTGTCAGATGGTCAGCACTGTGGAATTTGTGCCGCCTATGGCTTCTCTGGAGGAGAAA
  GGAATCCTGTTTACAAGTGATTACGTTTTGGCTAGCACCAACAGCTACACTATAAGCCCTCCAACAATCT
  CCGCCTCTGATGCACTGGCGCGGCGGTTTGCCTTCGACGTAGACTTCGAGGTAATGTCTCAGTACAATAA
  TAAGGGGCACCTGAACATGGCAACGGCAACTAAGGCCTGTACAGACTGTCATAAACCTGCCAATTTCAGT
  TCTTGCTGTCCTCTTGTCTGCGGGAAAGCAATCCAATTTGTGGACAAAATGACTAGAACCCGCTATACTG
  TCGACGAACTGACTACATTGATTGTTAATGAGAGAAACCGAAGGTACAATATTGGGAATTGTCTGGAGGC
  CCTTTTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100698 representing YP_003795181
  Red=Cloning sites Green=Tags
  
  MGDSWMKKFSDACNAAKGLEWISQKIGKFIDWIKTKILPEAQDKVEYLSKLKHLDTLENQVTTLHQSCPS
  QEDQETLFNNIRWLAIKARKFAPLYASEARRIFKMESTINNYIQFKTKHRIEPVCLLIHGTPGTGKSIAT
  GLIGRAIAKQANTSTYSLPPDPSHFDGYKQQGVVIMDDLNQNPDGEDMKLFCQMVSTVEFVPPMASLEEK
  GILFTSDYVLASTNSYTISPPTISASDALARRFAFDVDFEVMSQYNNKGHLNMATATKACTDCHKPANFS
  SCCPLVCGKAIQFVDKMTRTRYTVDELTTLIVNERNRRYNIGNCLEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795181
• RefSeq ORF: 990
• MW: 37.2 kDa