2A (NC_014336) Virus Tagged ORF Clone

CAT#: VC100696

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 109], codon optimized for human cell expression, YP_003795179

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100696 represents NCBI reference of YP_003795179 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTCGGCCACCAGAACAAGGCTATCTACGTCGCCGGATACAAGATTTGCAACTATCACCTGGCCA
  CACAGGAGGACATGGAACATGCCGTCGCAGTGATGTGGGACCGGGATCTGATGGTCGCTGAAAGTAGGGC
  ACAAGGCATAGATACCATCGCGCGCTGCAGTTGCAAGTGTGGCGTCTATTATTGCGAGTCTATGCGCAAG
  TACTACCCTGTGAGCGTTGAAGGACCTACCTTCCGATATATGGACGCAAATGAATATTACCCTGCGAGAT
  ACCAGAGCCACATGCTGATAGGAGCCGGGTTTGCCATGCCTGGCGACTGTGGCGGCATCCTCCGCTGCAC
  CCACGGTGTGATGGGCCTGATCACTGCCGGTGGCGAGGGGGTGGTCGCCTTTGCGGACGTCCGAGACCTC
  TTTGCTTATGAAGAAGAGGCAATGGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100696 representing YP_003795179
  Red=Cloning sites Green=Tags
  
  MGFGHQNKAIYVAGYKICNYHLATQEDMEHAVAVMWDRDLMVAESRAQGIDTIARCSCKCGVYYCESMRK
  YYPVSVEGPTFRYMDANEYYPARYQSHMLIGAGFAMPGDCGGILRCTHGVMGLITAGGEGVVAFADVRDL
  FAYEEEAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 450 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795179
• RefSeq ORF: 450
• MW: 16.7 kDa