VP3 (NC_014336) Virus Tagged ORF Clone

CAT#: VC100694

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 109], codon optimized for human cell expression, YP_003795177


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Reconstitution Protocol

USD 400.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol VP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100694 represents NCBI reference of YP_003795177 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTGCCTGTGCTGAACACTCCCGGCAGCAATCAGTTTCTCACTACCGACAATTTCCAATCACCAT
GTATCGTGCCAAACTATGACGTCACAGCTCCCATTCATATCCCTGGCGAAGTACGGAACATGATGGAGCT
GGCCGAAATCGATTCCGTGATTCCCATGAATGCCGTCGCCGGTAAAGTTAATACCATGGAAGCTTACCCA
ATTCCACTCAATCAGGGTACGCCCACAAAAGATCAATCAATCTTCAGCTTGAGCCTTTCACCTGCAAGTG
ACGAGCGCCTTTCCCGAACACTGCTCGGGGAGATCCTGAATTACTATACTCACTGGGCAGGAAGCATCAA
ATTTACTTTTCTGTTCTGTGGATCAATGATGGCAACGGGCAAGATACTCATTAGCTACTCTCCGCCTGGC
GCTGCACCACCTACCACACGCAAAGACGCCATGTTGGGTACTCACGTTATCTGGGATATCGGCCTGCAGT
CTTCCGTGACTCTGGTGGCTCCATGGATATCAAATACAGATTACCGGAGGTCCACTAAAGATGATTTCAC
CGAGGGGGGATACATTACTGCCTTTTACCAGACCGCCATCGTGGTGCCGGCCGGCGCTCCCAAGAGTATG
TCTATGCTTGCTTTCGTGTCCGCCTGCAATGACTTTTCCGCGCGGCTTTTGCGCGATACTCCATTCGTCC
GACAGCTGGCATCAGCCTGGAATCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100694 representing YP_003795177
Red=Cloning sites Green=Tags

MGLPVLNTPGSNQFLTTDNFQSPCIVPNYDVTAPIHIPGEVRNMMELAEIDSVIPMNAVAGKVNTMEAYP
IPLNQGTPTKDQSIFSLSLSPASDERLSRTLLGEILNYYTHWAGSIKFTFLFCGSMMATGKILISYSPPG
AAPPTTRKDAMLGTHVIWDIGLQSSVTLVAPWISNTDYRRSTKDDFTEGGYITAFYQTAIVVPAGAPKSM
SMLAFVSACNDFSARLLRDTPFVRQLASAWNQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_014336
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Reference Data
RefSeq NC_014336.1, YP_003795177
RefSeq ORF 726
MW 26.4 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.