VP3 (NC_014336) Virus Tagged ORF Clone

CAT#: VC100694

Myc-DDK-tagged ORF clone of viral ORF for VP3 [Human enterovirus 109], codon optimized for human cell expression, YP_003795177

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100694 represents NCBI reference of YP_003795177 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCCTGTGCTGAACACTCCCGGCAGCAATCAGTTTCTCACTACCGACAATTTCCAATCACCAT
  GTATCGTGCCAAACTATGACGTCACAGCTCCCATTCATATCCCTGGCGAAGTACGGAACATGATGGAGCT
  GGCCGAAATCGATTCCGTGATTCCCATGAATGCCGTCGCCGGTAAAGTTAATACCATGGAAGCTTACCCA
  ATTCCACTCAATCAGGGTACGCCCACAAAAGATCAATCAATCTTCAGCTTGAGCCTTTCACCTGCAAGTG
  ACGAGCGCCTTTCCCGAACACTGCTCGGGGAGATCCTGAATTACTATACTCACTGGGCAGGAAGCATCAA
  ATTTACTTTTCTGTTCTGTGGATCAATGATGGCAACGGGCAAGATACTCATTAGCTACTCTCCGCCTGGC
  GCTGCACCACCTACCACACGCAAAGACGCCATGTTGGGTACTCACGTTATCTGGGATATCGGCCTGCAGT
  CTTCCGTGACTCTGGTGGCTCCATGGATATCAAATACAGATTACCGGAGGTCCACTAAAGATGATTTCAC
  CGAGGGGGGATACATTACTGCCTTTTACCAGACCGCCATCGTGGTGCCGGCCGGCGCTCCCAAGAGTATG
  TCTATGCTTGCTTTCGTGTCCGCCTGCAATGACTTTTCCGCGCGGCTTTTGCGCGATACTCCATTCGTCC
  GACAGCTGGCATCAGCCTGGAATCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100694 representing YP_003795177
  Red=Cloning sites Green=Tags
  
  MGLPVLNTPGSNQFLTTDNFQSPCIVPNYDVTAPIHIPGEVRNMMELAEIDSVIPMNAVAGKVNTMEAYP
  IPLNQGTPTKDQSIFSLSLSPASDERLSRTLLGEILNYYTHWAGSIKFTFLFCGSMMATGKILISYSPPG
  AAPPTTRKDAMLGTHVIWDIGLQSSVTLVAPWISNTDYRRSTKDDFTEGGYITAFYQTAIVVPAGAPKSM
  SMLAFVSACNDFSARLLRDTPFVRQLASAWNQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795177
• RefSeq ORF: 726
• MW: 26.4 kDa