VP1 (NC_014336) Virus Tagged ORF Clone

CAT#: VC100695

Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human enterovirus 109], codon optimized for human cell expression, YP_003795178

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100695 represents NCBI reference of YP_003795178 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGTTGATGATAAAATTGATGCGTTGGTGGACGGAATGGTCAATTTGGCGATTGATACAGTGCAAA
  ATACTGTGGCATCAGGTCCCACACACTCTAAGGAGGTCCCCGCACTGACTGCAGTGGAAACAGGTGCTAC
  CAGCAAGGTGGTCCCCTCCGACCTTATAGAAACGCGACATGTAATCAATAAACGCCAACGGAGCGAGTGT
  ACTGTAGAGAGTTTTCTGGGCAGAGCAGCGCTCGTAACTATCCTTAAGCTCCAGAATGCCGCCCCTACAT
  CTGATAACGCTAAGGACCTCTTCGCGACCTGGAAGATCTCATATCACTACGCATACCAGCTTAGGAGGAA
  ACTGGAAATGTTTACCTATAGTCGCTTCGACATCGAACTTACCTTTGTCATAACTGAGAGGTTCGCCGCA
  AGCGGGGACGGTTCTAGCCGCCACTACGTCTATCAAATCATGTACATCCCCCCTGGAGCCCCCATCCCCC
  AAGCCTGGGATGATTTTACTTGGCAGACCTCAACAAACCCATCAGTATTCTATACCACCGGCAATGCTCC
  TCCCCGAGTATCTGTGCCTTTCATGGGAATCAGTGGAGCCTACAGCCACTTCTATGACGGCTTCTCTCTG
  GTACCCCACACTCAGTTTGACGCTGGGGCCACAAATAAATATGGCTACAACAGCATCAACGACTTCGGAA
  CCGTTGCTGTGAGGGTGGTGAACGAGCATGATCCAGTCGACATTCACGCTAAAGTCCGGGTGTACTTCAA
  GCCCAAACACATCCGGGTGTGGTGTCCTCGGCCCCCACGGGTTGTTGACTACACTGGTCCGTCCGTTGAC
  TACAAACAGACAAATAAAGTCATGACCCAAGTGGCCTCTATTCGAACCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100695 representing YP_003795178
  Red=Cloning sites Green=Tags
  
  MGVDDKIDALVDGMVNLAIDTVQNTVASGPTHSKEVPALTAVETGATSKVVPSDLIETRHVINKRQRSEC
  TVESFLGRAALVTILKLQNAAPTSDNAKDLFATWKISYHYAYQLRRKLEMFTYSRFDIELTFVITERFAA
  SGDGSSRHYVYQIMYIPPGAPIPQAWDDFTWQTSTNPSVFYTTGNAPPRVSVPFMGISGAYSHFYDGFSL
  VPHTQFDAGATNKYGYNSINDFGTVAVRVVNEHDPVDIHAKVRVYFKPKHIRVWCPRPPRVVDYTGPSVD
  YKQTNKVMTQVASIRTY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014336
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014336.1, YP_003795178
• RefSeq ORF: 891
• MW: 33.2 kDa