VP2 (1B) (NC_001612) Virus Tagged ORF Clone

CAT#: VC100717

Myc-DDK-tagged ORF clone of viral ORF for VP2 (1B) [Human enterovirus A], codon optimized for human cell expression, NP_740527

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP2 (1B)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100717 represents NCBI reference of NP_740527 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCTAGCGCCGAAGCTTGCGGGTATAGTGACAGGGTGGCACAGCTGACAATCGGAAATAGTACCA
  TCACTACGCAAGAAGCGGCCAATATTATTATTGCGTATGGTGAATGGCCGGAGTACTGCAAGGATGCCGA
  TGCTACGGCGGTCGACAAGCCGACTCGCCCCGACGTGTCCGTCAACAGATTCTTCACATTGGATACCAAA
  TCTTGGGCTAAAGATAGCAAGGGTTGGTACTGGAAGTTTCCCGACGTCCTCACTGAAGTCGGCGTGTTTG
  GGCAGAACGCCCAGTTTCACTATCTTTATAGGTCCGGCTTTTGCGTGCACGTGCAGTGTAATGCTTCCAA
  GTTCCACCAGGGCGCCCTGCTTGTCGCCATTCTCCCAGAGTACGTGTTGGGGACCATCGCTGGCGGCGAT
  GGCAACGAGAATAGCCACCCCCCATACGTCACCACCCAGCCCGGCCAGGTAGGGGCAGTGTTGACTAATC
  CCTATGTCCTCGACGCTGGCGTTCCTCTGTCCCAGCTGACAGTGTGCCCCCACCAGTGGATTAATCTGCG
  AACAAACAACTGTGCCACAATCATAGTGCCATACATGAACACTGTTCCATTCGATTCCGCACTTAATCAT
  TGTAATTTCGGGCTGATCGTGGTGCCAGTGGTGCCACTGGATTTTAACGCTGGGGCCACATCCGAAATTC
  CTATAACCGTAACAATCGCTCCCATGTGCGCCGAATTTGCTGGACTGCGCCAGGCGATCAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100717 representing NP_740527
  Red=Cloning sites Green=Tags
  
  MSPSAEACGYSDRVAQLTIGNSTITTQEAANIIIAYGEWPEYCKDADATAVDKPTRPDVSVNRFFTLDTK
  SWAKDSKGWYWKFPDVLTEVGVFGQNAQFHYLYRSGFCVHVQCNASKFHQGALLVAILPEYVLGTIAGGD
  GNENSHPPYVTTQPGQVGAVLTNPYVLDAGVPLSQLTVCPHQWINLRTNNCATIIVPYMNTVPFDSALNH
  CNFGLIVVPVVPLDFNAGATSEIPITVTIAPMCAEFAGLRQAIKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740527
• RefSeq ORF: 765
• MW: 27.7 kDa