2A (NC_001612) Virus Tagged ORF Clone

CAT#: VC100720

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus A], codon optimized for human cell expression, NP_740530

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100720 represents NCBI reference of NP_740530 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGTTCGGGCAGCAGTCCGGAGCAATCTATGTGGGGAACTACAGAGTAGTGAACCGACATCTGG
  CTACACATAATGACTGGGCCAATCTTGTTTGGGAGGACTCATCACGGGACCTTCTGGTAAGTAGCACCAC
  CGCCCAGGGGTGTGACACCATTGCAAGGTGTGATTGTCAGACAGGAGTCTATTACTGTTCTTCAAGGCGG
  AAACATTACCCCGTGTCTTTTTCCAAACCATCTCTGATCTTCGTGGAGGCATCCGAATACTATCCTGCCC
  GCTATCAGTCTCACCTCATGCTCGCCGTGGGACACTCCGAACCTGGGGACTGTGGCGGGATCCTGCGATG
  TCAGCACGGAGTCGTGGGCATTGTGTCAACCGGCGGCAACGGCCTCGTGGGCTTTGCTGACGTACGGGAT
  CTGCTCTGGCTTGATGAAGAGGCCATGGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100720 representing NP_740530
  Red=Cloning sites Green=Tags
  
  MGKFGQQSGAIYVGNYRVVNRHLATHNDWANLVWEDSSRDLLVSSTTAQGCDTIARCDCQTGVYYCSSRR
  KHYPVSFSKPSLIFVEASEYYPARYQSHLMLAVGHSEPGDCGGILRCQHGVVGIVSTGGNGLVGFADVRD
  LLWLDEEAMEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 453 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740530
• RefSeq ORF: 453
• MW: 16.7 kDa