VP1 (1D) (NC_001612) Virus Tagged ORF Clone

CAT#: VC100719

Myc-DDK-tagged ORF clone of viral ORF for VP1 (1D) [Human enterovirus A], codon optimized for human cell expression, NP_740529

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP1 (1D)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100719 represents NCBI reference of NP_740529 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACGGGATCGCAGATATGATAGATCAAGCCGTCACATCAAGGGTCGGAAGGGCTCTGACTTCCC
  TCCAAGTGGAGCCAACCGCAGCTAACACAAATGCCTCAGAGCACCGGCTCGGCACCGGCCTGGTGCCGGC
  TCTGCAGGCTGCCGAAACTGGAGCATCTAGTAATGCCCAGGATGAGAATCTGATTGAGACAAGATGTGTA
  CTCAACCATCACTCCACTCAGGAGACGACCATCGGGAACTTCTTCTCCAGGGCCGGCCTCGTAAGCATCA
  TCACGATGCCCACGACAGGGACGCAGAACACGGACGGTTACGTGAATTGGGATATCGACCTGATGGGATA
  CGCACAAATGCGACGCAAATGTGAGCTCTTTACCTACATGCGGTTCGACGCAGAGTTTACATTTGTCGCC
  GCCAAGCCTAACGGCGAGTTGGTGCCTCAACTTCTTCAGTATATGTACGTGCCACCCGGCGCTCCAAAGC
  CAACCAGCAGAGACTCCTTTGCGTGGCAGACAGCAACAAACCCCAGCATCTTTGTGAAGCTTACTGATCC
  ACCTGCTCAGGTGTCCGTCCCCTTTATGAGCCCGGCATCAGCGTATCAGTGGTTCTACGATGGATATCCG
  ACCTTCGGGGCTCATCCTCAATCTAATGATGCCGACTATGGTCAGTGCCCAAATAACATGATGGGCACCT
  TCTCAATTAGAACCGTCGGTACTGAAAAGTCACCCCACAGTATCACACTGAGGGTGTACATGAGGATCAA
  GCACGTAAGAGCTTGGATCCCGCGCCCACTGAGGAATCAACCATATCTGTTCAAAACGAACCCCAACTAT
  AAGGGAAACGACATTAAATGCACCAGCACTAGCCGGGATAAGATAACCACCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100719 representing NP_740529
  Red=Cloning sites Green=Tags
  
  MGDGIADMIDQAVTSRVGRALTSLQVEPTAANTNASEHRLGTGLVPALQAAETGASSNAQDENLIETRCV
  LNHHSTQETTIGNFFSRAGLVSIITMPTTGTQNTDGYVNWDIDLMGYAQMRRKCELFTYMRFDAEFTFVA
  AKPNGELVPQLLQYMYVPPGAPKPTSRDSFAWQTATNPSIFVKLTDPPAQVSVPFMSPASAYQWFYDGYP
  TFGAHPQSNDADYGQCPNNMMGTFSIRTVGTEKSPHSITLRVYMRIKHVRAWIPRPLRNQPYLFKTNPNY
  KGNDIKCTSTSRDKITTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 894 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740529
• RefSeq ORF: 894
• MW: 33.1 kDa