3A (NC_001612) Virus Tagged ORF Clone

CAT#: VC100723

Myc-DDK-tagged ORF clone of viral ORF for 3A [Human enterovirus A], codon optimized for human cell expression, NP_740533

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100723 represents NCBI reference of NP_740533 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCACCCAAATTTCGGCCCATTCGGATCTCACTGGAAGAAAAACCCGCTCCTGATGCTATTTCAG
  ACCTGCTGGCCTCTGTGGACTCTGAAGAGGTCCGGCAATATTGTAGAGATCAGGGATGGATCATTCCAGA
  GACGCCCACAAATGTGGAACGGCACCTGAACAGAGCAGTGCTGATTATGCAGTCCATCGCCACAGTGGTC
  GCAGTCGTGTCCCTGGTGTACGTTATCTACAAGTTGTTCGCCGGCTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100723 representing NP_740533
  Red=Cloning sites Green=Tags
  
  MGPPKFRPIRISLEEKPAPDAISDLLASVDSEEVRQYCRDQGWIIPETPTNVERHLNRAVLIMQSIATVV
  AVVSLVYVIYKLFAGFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 261 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740533
• RefSeq ORF: 261
• MW: 9.8 kDa