2C (NC_001612) Virus Tagged ORF Clone

CAT#: VC100722

Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus A], codon optimized for human cell expression, NP_740532

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100722 represents NCBI reference of NP_740532 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCAGTTGGTTGAAGAAGTTTAATGATATGGCTAATGCCGCCAAGGGTCTGGAGTGGATCTCTA
  ACAAAATCTCCAAGTTTATCGACTGGCTGAAGGAAAAAATAATCCCCGCTGCTAAAGAGAAGGTAGAGTT
  CCTGAATAACCTGAAGCAGCTTCCCCTGCTGGAGAACCAAATTTCAAACCTGGAACAGAGCGCGGCCTCA
  CAGGAGGATCTGGAGGCTATGTTCGGAAACGTGAGCTACCTCGCGCACTTTTGCCGGAAATTCCAGCCCC
  TCTATGCCACCGAAGCCAAAAGAGTTTACGCTCTGGAAAAGCGCATGAACAACTATATGCAGTTCAAATC
  CAAACACCGGATCGAGCCTGTTTGCCTGATTATCAGGGGAAGTCCTGGAACGGGTAAATCATTGGCCACA
  GGAATTATAGCTAGAGCTATTGCCGATAAGTACCATTCAAGTGTCTATAGCCTTCCACCGGATCCCGACC
  ACTTTGACGGCTATAAACAACAGGTGGTAACAGTCATGGACGATCTTTGCCAGAATCCTGACGGCAAAGA
  CATGAGTCTGTTTTGTCAGATGGTTTCCACTGTGGATTTCATCCCGCCGATGGCGTCCCTGGAGGAGAAG
  GGTGTGTCATTTACTAGCAAGTTTGTGATAGCCTCCACCAACGCCAGCAACATCATCGTACCCACAGTTA
  GTGACTCAGACGCCATCCGCCGGCGATTTTATATGGACTGTGACATTGAGGTCACCGACAGTTACAAGAC
  CGACTTGGGGCGCCTCGATGCGGGCCGGGCCGCTAGACTGTGTAGTGAGAATAATACGGCCAACTTCAAA
  CGGTGTTCACCCCTTGTTTGTGGCAAGGCTATTCAGCTGAGGGACAGAAAGAGTAAAGTGCGATACTCCG
  TTGATACCGTGGTATCTGAGCTGATTAGAGAGTACAATAATAGATACGCTATTGGTAATACAATAGAAGC
  CCTCTTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100722 representing NP_740532
  Red=Cloning sites Green=Tags
  
  MSASWLKKFNDMANAAKGLEWISNKISKFIDWLKEKIIPAAKEKVEFLNNLKQLPLLENQISNLEQSAAS
  QEDLEAMFGNVSYLAHFCRKFQPLYATEAKRVYALEKRMNNYMQFKSKHRIEPVCLIIRGSPGTGKSLAT
  GIIARAIADKYHSSVYSLPPDPDHFDGYKQQVVTVMDDLCQNPDGKDMSLFCQMVSTVDFIPPMASLEEK
  GVSFTSKFVIASTNASNIIVPTVSDSDAIRRRFYMDCDIEVTDSYKTDLGRLDAGRAARLCSENNTANFK
  RCSPLVCGKAIQLRDRKSKVRYSVDTVVSELIREYNNRYAIGNTIEALFQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740532
• RefSeq ORF: 990
• MW: 37.3 kDa