2B (NC_001612) Virus Tagged ORF Clone

CAT#: VC100721

Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus A], codon optimized for human cell expression, NP_740531

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100721 represents NCBI reference of NP_740531 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCAGCGACTACATTAAGGGTCTGGGAGATGCTTTCGGCACCGGTTTTACGGATGCTGTTTCTA
  GGGAAGTGGAAGCCCTGAAGAACCATCTCATCGGAAGTGAAGGCGCTGTTGAGAAGATCCTGAAGAACCT
  GATTAAACTGATTTCCGCCCTGGTGATCGTCATCCGGTCTGATTATGACATGGTCACTTTGACGGCCACA
  CTCGCGCTGATTGGATGTCACGGCTCTCCTTGGGCCTGGATTAAAGCTAAGACCGCCTCTATACTGGGCA
  TACCTATTGCCCAGAAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100721 representing NP_740531
  Red=Cloning sites Green=Tags
  
  MGVSDYIKGLGDAFGTGFTDAVSREVEALKNHLIGSEGAVEKILKNLIKLISALVIVIRSDYDMVTLTAT
  LALIGCHGSPWAWIKAKTASILGIPIAQKQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740531
• RefSeq ORF: 300
• MW: 10.6 kDa