3C (NC_001612) Virus Tagged ORF Clone

CAT#: VC100725

Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus A], codon optimized for human cell expression, NP_740535

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 3C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100725 represents NCBI reference of NP_740535 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCCTCCCTCGATTTCGCGCTCTCACTGCTCAGACGCAATATTAGACAGGTGCAGACAGACCAGG
  GCCACTTTACAATGCTGGGCGTGAGGGATAGGCTGGCTGTGCTGCCTAGACACTCTCAGCCTGGCAAGAC
  CATCTGGGTGGAGCACAAACTCATTAACATCCTCGATGCTGTGGAGCTGGTAGATGAGCAGGGAGTGAAT
  CTCGAGCTCACCCTCGTGACACTGGACACGAACGAGAAGTTTAGGGACATTACGAAATTTATCCCTGAAA
  ATATATCCGCAGCAAGTGACGCCACACTTGTGATCAACACCGAACATATGCCTTCCATGTTTGTCCCCGT
  GGGCGACGTGGTTCAATACGGCTTTCTGAACCTGTCAGGCAAACCTACGCACAGAACGATGATGTACAAC
  TTCCCTACCAAAGCCGGTCAATGTGGCGGTGTTGTGACTAGCGTTGGGAAGGTAATCGGCATCCACATTG
  GTGGAAATGGGAGACAGGGGTTTTGTGCAGGCCTGAAACGATCTTACTTCGCGAGCGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100725 representing NP_740535
  Red=Cloning sites Green=Tags
  
  MGPSLDFALSLLRRNIRQVQTDQGHFTMLGVRDRLAVLPRHSQPGKTIWVEHKLINILDAVELVDEQGVN
  LELTLVTLDTNEKFRDITKFIPENISAASDATLVINTEHMPSMFVPVGDVVQYGFLNLSGKPTHRTMMYN
  FPTKAGQCGGVVTSVGKVIGIHIGGNGRQGFCAGLKRSYFASEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 552 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740535
• RefSeq ORF: 552
• MW: 20.3 kDa