VP3 (1C) (NC_001612) Virus Tagged ORF Clone

CAT#: VC100718

Myc-DDK-tagged ORF clone of viral ORF for VP3 (1C) [Human enterovirus A], codon optimized for human cell expression, NP_740528

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VP3 (1C)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100718 represents NCBI reference of NP_740528 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCCCCACCGAGCTGAAGCCTGGTACCAATCAGTTCCTGACAACTGACGATGGCGTTTCTGCAC
  CAATCCTTCCCGGCTTCCACCCCACACCCGCAATCCATATACCGGGCGAGGTGCGGAACCTGCTGGAGAT
  ATGCCGGGTAGAAACGATTCTCGAGGTAAACAATCTTCAGAGCAACGAGACAACACCAATGCAGAGGCTC
  TGTTTTCCCGTTTCCGTTCAGTCAAAAACCGGCGAGCTGTGCGCAGTATTCCGAGCGGACCCTGGCCGCA
  ATGGGCCATGGCAGTCCACAATCCTTGGTCAGCTGTGCCGATACTACACTCAGTGGTCTGGCTCTCTCGA
  AGTGACTTTTATGTTCGCAGGGTCTTTTATGGCTACAGGCAAAATGCTTATTGCCTACACCCCTCCTGGT
  GGGGGTGTGCCCGCCGACCGCCTTACTGCAATGCTGGGCACTCATGTCATTTGGGATTTTGGCTTGCAGA
  GCAGCGTGACTCTGGTGATCCCATGGATCTCTAATACACATTATAGGGCTCACGCCAAAGACGGATATTT
  CGACTACTATACGACCGGTACTATTACCATTTGGTACCAGACCAACTATGTAGTTCCCATTGGAGCACCC
  ACTACCGCTTATATTGTCGCGCTTGCCGCCGCCCAGGACAACTTCACCATGAAACTTTGCAAGGACACAG
  AGGACATTGAACAGTCTGCCAACATTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100718 representing NP_740528
  Red=Cloning sites Green=Tags
  
  MGIPTELKPGTNQFLTTDDGVSAPILPGFHPTPAIHIPGEVRNLLEICRVETILEVNNLQSNETTPMQRL
  CFPVSVQSKTGELCAVFRADPGRNGPWQSTILGQLCRYYTQWSGSLEVTFMFAGSFMATGKMLIAYTPPG
  GGVPADRLTAMLGTHVIWDFGLQSSVTLVIPWISNTHYRAHAKDGYFDYYTTGTITIWYQTNYVVPIGAP
  TTAYIVALAAAQDNFTMKLCKDTEDIEQSANIQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001612
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001612.1, NP_740528
• RefSeq ORF: 729
• MW: 26.7 kDa